BMRB Entry 18470
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18470
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Title: Cu(I) form gt CsoR PubMed: 23001947
Deposition date: 2012-05-17 Original release date: 2019-08-30
Authors: Coyne, Harold; Giedroc, David; Kester, Benjamin
Citation: Coyne, Harold; Giedroc, David. "Backbone resonance assignments of the homotetrameric (48 kD) copper sensor CsoR from Geobacillus thermodenitrificans in the apo- and Cu(I)-bound states: insights into copper-mediated allostery" Biomol. NMR Assign. 7, 279-283 (2013).
Assembly members:
gt_CsoR, polymer, 105 residues, Formula weight is not available
entity_CU1, non-polymer, 63.546 Da.
Natural source: Common Name: Geobacillus thermodenitrifcans Taxonomy ID: 33940 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus thermodenitrifcans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 15b
Entity Sequences (FASTA):
gt_CsoR: MAHPSQEEHVLHGTMIPRTK
EEIENIMKRLKRIEGQVRGV
QKMVEDNRYCIDILVQISAI
QAALRQVGMQLLERHANHCV
AKAIREGSGEQSLRELMDVI
KQFAK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 297 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 98 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cu(I) gt CsoR, 1 | 1 |
| 2 | Cu(I) gt CsoR, 2 | 1 |
| 3 | Cu(I) gt CsoR, 3 | 1 |
| 4 | Cu(I) gt CsoR, 4 | 1 |
| 5 | Cu(I), 1 | 2 |
| 6 | Cu(I), 2 | 2 |
| 7 | Cu(I), 3 | 2 |
| 8 | Cu(I), 4 | 2 |
Entities:
Entity 1, Cu(I) gt CsoR, 1 105 residues - Formula weight is not available
| 1 | MET | ALA | HIS | PRO | SER | GLN | GLU | GLU | HIS | VAL | ||||
| 2 | LEU | HIS | GLY | THR | MET | ILE | PRO | ARG | THR | LYS | ||||
| 3 | GLU | GLU | ILE | GLU | ASN | ILE | MET | LYS | ARG | LEU | ||||
| 4 | LYS | ARG | ILE | GLU | GLY | GLN | VAL | ARG | GLY | VAL | ||||
| 5 | GLN | LYS | MET | VAL | GLU | ASP | ASN | ARG | TYR | CYS | ||||
| 6 | ILE | ASP | ILE | LEU | VAL | GLN | ILE | SER | ALA | ILE | ||||
| 7 | GLN | ALA | ALA | LEU | ARG | GLN | VAL | GLY | MET | GLN | ||||
| 8 | LEU | LEU | GLU | ARG | HIS | ALA | ASN | HIS | CYS | VAL | ||||
| 9 | ALA | LYS | ALA | ILE | ARG | GLU | GLY | SER | GLY | GLU | ||||
| 10 | GLN | SER | LEU | ARG | GLU | LEU | MET | ASP | VAL | ILE | ||||
| 11 | LYS | GLN | PHE | ALA | LYS |
Entity 2, Cu(I), 1 - Cu - 63.546 Da.
| 1 | CU1 |
Samples:
sample_1: Cu(I) gt CsoR, [U-100% 13C; U-100% 15N; U-70% 2H], 0.4 – 0.8 mM; Cu(I) ion0.4 – 0.8 mM; NaCl 115 mM; TCEP 5 mM; EDTA 3 mM; ARG 20 mM; GLU 20 mM
sample_conditions_1: ionic strength: 0.115 M; pH: 6.0; pressure: 1 atm; temperature: 322 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts