BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18492

Title: Solution structure of the SANT2 domain of the human nuclear receptor corepressor 2 (NCoR2), Northeast Structural Genomics Consortium (NESG) target ID HR4636E.

Deposition date: 2012-05-30 Original release date: 2012-06-18

Authors: Montecchio, Meri; Lemak, Alexander; Yee, Adelinda; Xu, Chao; Garcia, Maite; Houliston, Scott; Bellanda, Massimo; Min, Jinrong; Arrowsmith, Cheryl; Montelione, G.T.

Citation: Montecchio, Meri; Lemak, Alexander; Yee, Adelinda; Xu, Chao; Garcia, Maite; Houliston, Scott; Bellanda, Massimo; Min, Jinrong; Arrowsmith, Cheryl. "Solution structure of the SANT2 domain of the human nuclear receptor corepressor 2 (NCoR2)."  .

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28-MHL

Entity Sequences (FASTA):
entity_1: MHHHHHHSSGRENLYFQGWT EEEMGTAKKGLLEHGRNWSA IARMVGSKTVSQCKNFYFNY KKRQNLDEILQQHKLKMEKE RNARRKKKK

Data typeCount
13C chemical shifts287
15N chemical shifts71
1H chemical shifts466

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SANT2 domain of NCoR21

Entities:

Entity 1, SANT2 domain of NCoR2 89 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   ARGGLUASNLEUTYRPHEGLNGLYTRPTHR
3   GLUGLUGLUMETGLYTHRALALYSLYSGLY
4   LEULEUGLUHISGLYARGASNTRPSERALA
5   ILEALAARGMETVALGLYSERLYSTHRVAL
6   SERGLNCYSLYSASNPHETYRPHEASNTYR
7   LYSLYSARGGLNASNLEUASPGLUILELEU
8   GLNGLNHISLYSLEULYSMETGLULYSGLU
9   ARGASNALAARGARGLYSLYSLYSLYS

Samples:

sample_1: SANT2, [U-13C; U-15N], 1 mM; sodium phosphate 25 mM; NaCl 200 mM; ZnSO4 0.01 mM; DTT 10 mM; benzamidine 1 mM; NaN3 0.01%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - peak picking

MDDGUI, Gutmanas A., Orekhov V. - processing

FMCGUI, Lemak A., Steren C., Llinas M., Arrowsmith C. - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS, Bhattacharya and Montelione - structure validation

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAE34400
GB EPY89720 KQK78591 KQL90039 KQL90040 KQL90041
REF XP_005193418 XP_005612752 XP_007539833 XP_007665136 XP_008111869

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts