BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18505

Title: Backbone and side chain assignments of MHV N protein NTD (aa60-197)

Deposition date: 2012-06-06 Original release date: 2012-07-10

Authors: Keane, Sarah; Giedroc, David

Citation: Keane, Sarah; Giedroc, David. "Backbone and side chain assignments of MHV N NTD"  The BMRB entry is the only known published source for the data..

Assembly members:
MHV_N_NTD, polymer, 138 residues, Formula weight is not available

Natural source:   Common Name: Murine Hepatitis Virus   Taxonomy ID: 11138   Superkingdom: Virus   Kingdom: not available   Genus/species: Betacoronavirus Murine coronavirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMBP

Entity Sequences (FASTA):
MHV_N_NTD: GSVVPHYSWFSGITQFQKGK EFQFAEGQGVPIANGIPASE QKGYWYRHNRRSFKTPDGQQ KQLLPRWYFYYLGTGPHAGA SYGDSIEGVFWVANSQADTN TRSDIVERDPSSHEAIPTRF APGTVLPQGFYVEGSGRS

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts122
1H chemical shifts694

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD1

Entities:

Entity 1, NTD 138 residues - Formula weight is not available

1   GLYSERVALVALPROHISTYRSERTRPPHE
2   SERGLYILETHRGLNPHEGLNLYSGLYLYS
3   GLUPHEGLNPHEALAGLUGLYGLNGLYVAL
4   PROILEALAASNGLYILEPROALASERGLU
5   GLNLYSGLYTYRTRPTYRARGHISASNARG
6   ARGSERPHELYSTHRPROASPGLYGLNGLN
7   LYSGLNLEULEUPROARGTRPTYRPHETYR
8   TYRLEUGLYTHRGLYPROHISALAGLYALA
9   SERTYRGLYASPSERILEGLUGLYVALPHE
10   TRPVALALAASNSERGLNALAASPTHRASN
11   THRARGSERASPILEVALGLUARGASPPRO
12   SERSERHISGLUALAILEPROTHRARGPHE
13   ALAPROGLYTHRVALLEUPROGLNGLYPHE
14   TYRVALGLUGLYSERGLYARGSER

Samples:

sample_1: MHV N NTD, [U-100% 13C; U-100% 15N], 200 uM; H2O 90%; D2O 10%

sample_2: MHV N NTD, [U-100% 13C; U-100% 15N], 200 uM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
HBCBCGCDHDsample_1isotropicsample_conditions_1
HBCBCGCDCEHEsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian VNMRS 600 MHz
  • Varian VNMRS 800 MHz

Related Database Links:

PDB
EMBL CAA25198
GB AAA46444 AAA46447 AAA46462 AAA74734 AAA76578
REF NP_045302
SP P03416 P18447 Q83360 Q9WCD0
AlphaFold Q9WCD0 P18447 P03416 Q83360

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts