BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18590

Title: S. aureus pepA1 NMR structure   PubMed: 23129767

Deposition date: 2012-07-11 Original release date: 2014-04-22

Authors: Sayed, N.; Nonin-Lecomte, Sylvie; Rety, S.; Felden, B.

Citation: Sayed, Nour; Nonin-Lecomte, Sylvie; Rety, Stephane; Felden, Brice. "Functional and structural insights of a Staphylococcus aureus apoptotic-like membrane peptide from a toxin-antitoxin module."  J. Biol. Chem. 287, 43454-43463 (2012).

Assembly members:
PEPA1, polymer, 30 residues, 3459.2068 Da.

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PEPA1: MLIFVHIIAPVISGCAIAFF SYWLSRRNTK

Data sets:
Data typeCount
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PEPA11

Entities:

Entity 1, PEPA1 30 residues - 3459.2068 Da.

1   METLEUILEPHEVALHISILEILEALAPRO
2   VALILESERGLYCYSALAILEALAPHEPHE
3   SERTYRTRPLEUSERARGARGASNTHRLYS

Samples:

sample_1: PEPA1 5 mM

sample_conditions_1: ionic strength: 0.0 mM; pH: 3.800; pressure: 1.000 atm; temperature: 288.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
COSYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, Sen et al. - collection

TOPSPIN vany, Bruker Biospin - collection

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

Analysis vany, CCPN - chemical shift assignment

CNS v2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz