BMRB Entry 18616
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18616
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Title: Relaxation rates for accurate spectral density mapping for unbiased analysis of nucleic acid motions probed by carbon-13 NMR relaxation PubMed: 27003380
Deposition date: 2012-07-26 Original release date: 2019-08-30
Authors: Kaderavek, Pavel; Zapletal, Vojtech; Fiala, Radovan; Sklenar, Vladimir; Zidek, Lukas
Citation: Kaderavek, Pavel; Zapletal, Vojtech; Fiala, Radovan; Srb, Pavel; Padrta, Petr; Precechtelova, Jana Pavlikova; Soltesova, Maria; Kowalewski, Jozef; Widmalm, Goran; Chmelik, Josef; Sklenar, Vladimir; Zidek, Lukas. "Spectral density mapping at multiple magnetic fields suitable for (13)C NMR relaxation studies" J. Magn. Reson. 266, 23-40 (2016).
Assembly members:
UUCG_tetraloop, polymer, 14 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
UUCG_tetraloop: GGCACUUCGGUGCC
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T1rho_relaxation
Data type | Count |
T1 relaxation values | 39 |
T1rho relaxation values | 39 |
dipole-CSA cross correlation relaxation values | 73 |
heteronuclear NOE values | 52 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UUCG teraloop | 1 |
Entities:
Entity 1, UUCG teraloop 14 residues - Formula weight is not available
1 | G | G | C | A | C | U | U | C | G | G | ||||
2 | U | G | C | C |
Samples:
sample_1: UUCG tetraloop, [U-13C; U-15N], 3.0 mM; EDTA 0.2 mM; sodium phosphate 10 mM; sodium azide 0.05%
sample_conditions_1: pH*: 6.7; pressure: 1 atm; temperature: 298.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D R1 13C,1H correlation | sample_1 | isotropic | sample_conditions_1 |
2D R1rho 13C,1H correlation | sample_1 | isotropic | sample_conditions_1 |
2D NOE 13C,1H correlation | sample_1 | isotropic | sample_conditions_1 |
2D constant-time 13C(coupled),1H correlation | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian Unity 400 MHz
- Varian Unity 500 MHz
- Varian Unity 600 MHz