BMRB Entry 18693

Name: 1H, 13C, and 15N resonance assignments of mouse peptide ESP4
Published: 2013-02-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18693

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of mouse peptide ESP4

Deposition date:

2012-08-31

Original release date:

2013-02-12

Authors:

Taniguchi, Masahiro

Citation:

Citation: Taniguchi, Masahiro; Yoshinaga, Sosuke; Haga-Yamanaka, Sachiko; Touhara, Kazushige; Terasawa, Hiroaki. "Backbone and side-chain H, 15N and 13C assignments of mouse peptide ESP4." Biomol. NMR Assignments 8, 7-9 (2014).
PubMed: 23179060

Assembly members:

Assembly members:
ESP4, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: House Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ESP4: GSGRVLTQTGKETTMSADHK TNHKADLEKNDSQGERNTQE AFEMILCAFNQEKMLLKDQA NSGQHELKLSKFFTALSKCG AQNYQVDTVNYRIIPHIYPL HSPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	410
15N chemical shifts	100
1H chemical shifts	645

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Exocrine gland-secreting peptide 4	1

Entities:

Entity 1, Exocrine gland-secreting peptide 4 104 residues - Formula weight is not available

1

GLY

SER

GLY

ARG

VAL

LEU

THR

GLN

THR

GLY

2

LYS

GLU

THR

MET

SER

ALA

ASP

HIS

LYS

3

THR

ASN

HIS

LYS

ALA

ASP

LEU

GLU

LYS

ASN

4

ASP

SER

GLN

GLY

GLU

ARG

ASN

THR

GLN

GLU

5

ALA

PHE

GLU

MET

ILE

LEU

CYS

ALA

PHE

ASN

6

GLN

GLU

LYS

MET

LEU

LYS

ASP

GLN

ALA

7

ASN

SER

GLY

GLN

HIS

GLU

LEU

LYS

LEU

SER

8

LYS

PHE

THR

ALA

LEU

SER

LYS

CYS

GLY

9

ALA

GLN

ASN

TYR

GLN

VAL

ASP

THR

VAL

ASN

10

TYR

ARG

ILE

PRO

HIS

ILE

TYR

PRO

LEU

11

HIS

SER

PRO

LYS

Samples:

sample_1: ESP4, [U-100% 13C; U-100% 15N], 100 uM; H2O 90%; D2O 10%

sample_2: ESP4, [U-100% 15N], 100 uM; H2O 90%; D2O 10%

sample_3: ESP4, [U-100% 13C; U-100% 15N], 100 uM; D2O 100%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection, peak picking, processing

Olivia, Yokochi, Sekiguchi and Inagaki - chemical shift assignment, chemical shift calculation, data analysis

TOPSPIN, Bruker Biospin - collection, data analysis

NMR spectrometers:

Bruker Avance 600 MHz

DBJ	BAF92721
REF	NP_001171054