BMRB Entry 18774

Name: Backbone 1H-13C-15N NMR assignments of yeast OMP synthase
Published: 2013-01-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18774

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H-13C-15N NMR assignments of yeast OMP synthase

Deposition date:

2012-10-10

Original release date:

2013-01-29

Authors:

Harris, Richard; Hansen, Michael; Barr, Eric; Cheng, Hong; Grubmeyer, Charles

Citation:

Citation: Hansen, Michael Riis; Harris, Richard; Barr, Eric; Cheng, Hong; Girvin, Mark; Grubmeyer, Charles. "Backbone (1)H, (13)C, (15)N NMR assignments of yeast OMP synthase in unliganded form and in complex with orotidine 5'-monophosphate." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23315339

Assembly members:

Assembly members:
oprtase, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
oprtase: PIMLEDYQKNFLELAIECQA LRFGSFKLKSGRESPYFFNL GLFNTGKLLSNLATAYAIAI IQSDLKFDVIFGPAYKGIPL AAIVCVKLAEIGGSKFQNIQ YAFNRKEAKDHGEGGIIVGS ALENKRILIIDDVMTAGTAI NEAFEIISNAKGQVVGSIIA LDRQEVVSTDDKEGLSATQT VSKKYGIPVLSIVSLIHIIT YLEGRITAEEKSKIEQYLQT YGASAENLYFQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	656
15N chemical shifts	212
1H chemical shifts	212

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	omp synthase, 1	1
2	omp synthase, 2	1

Entities:

Entity 1, omp synthase, 1 231 residues - Formula weight is not available

1

PRO

ILE

MET

LEU

GLU

ASP

TYR

GLN

LYS

ASN

2

PHE

LEU

GLU

LEU

ALA

ILE

GLU

CYS

GLN

ALA

3

LEU

ARG

PHE

GLY

SER

PHE

LYS

LEU

LYS

SER

4

GLY

ARG

GLU

SER

PRO

TYR

PHE

ASN

LEU

5

GLY

LEU

PHE

ASN

THR

GLY

LYS

LEU

SER

6

ASN

LEU

ALA

THR

ALA

TYR

ALA

ILE

ALA

ILE

7

ILE

GLN

SER

ASP

LEU

LYS

PHE

ASP

VAL

ILE

8

PHE

GLY

PRO

ALA

TYR

LYS

GLY

ILE

PRO

LEU

9

ALA

ILE

VAL

CYS

VAL

LYS

LEU

ALA

GLU

10

ILE

GLY

SER

LYS

PHE

GLN

ASN

ILE

GLN

11

TYR

ALA

PHE

ASN

ARG

LYS

GLU

ALA

LYS

ASP

12

HIS

GLY

GLU

GLY

ILE

VAL

GLY

SER

13

ALA

LEU

GLU

ASN

LYS

ARG

ILE

LEU

ILE

14

ASP

VAL

MET

THR

ALA

GLY

THR

ALA

ILE

15

ASN

GLU

ALA

PHE

GLU

ILE

SER

ASN

ALA

16

LYS

GLY

GLN

VAL

GLY

SER

ILE

ALA

17

LEU

ASP

ARG

GLN

GLU

VAL

SER

THR

ASP

18

ASP

LYS

GLU

GLY

LEU

SER

ALA

THR

GLN

THR

19

VAL

SER

LYS

TYR

GLY

ILE

PRO

VAL

LEU

20

SER

ILE

VAL

SER

LEU

ILE

HIS

ILE

THR

21

TYR

LEU

GLU

GLY

ARG

ILE

THR

ALA

GLU

22

LYS

SER

LYS

ILE

GLU

GLN

TYR

LEU

GLN

THR

23

TYR

GLY

ALA

SER

ALA

GLU

ASN

LEU

TYR

PHE

24

GLN

Samples:

sample_1: oprtase, [U-13C; U-15N; U-2H], 0.9 mM; PIPES 80 mM; DTT 1 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_2
3D HNCA	sample_1	isotropic	sample_conditions_2
3D HN(CO)CA	sample_1	isotropic	sample_conditions_2
3D HN(CA)CO	sample_1	isotropic	sample_conditions_2
3D HNCACB	sample_1	isotropic	sample_conditions_2
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_2

Software:

VNMRJ v2.2D, Varian - collection

TOPSPIN v3.1, Bruker Biospin - collection

NMRPipe v7.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Kazimierczuk - collection, processing

CCPN v2.2, CCPN - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Varian INOVA 600 MHz
Bruker Avance 900 MHz

BMRB	18775
PDB	2PRY 2PRZ 2PS1
DBJ	GAA25343
EMBL	CAA32901 CAA46665 CAA89112 CAY81712
GB	AAT93179 AHY76362 AJP40607 AJS61787 AJS62213
REF	NP_013601
SP	P13298
TPG	DAA09792
AlphaFold	P13298