BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18774

Title: Backbone 1H-13C-15N NMR assignments of yeast OMP synthase   PubMed: 23315339

Deposition date: 2012-10-10 Original release date: 2013-01-29

Authors: Harris, Richard; Hansen, Michael; Barr, Eric; Cheng, Hong; Grubmeyer, Charles

Citation: Hansen, Michael Riis; Harris, Richard; Barr, Eric; Cheng, Hong; Girvin, Mark; Grubmeyer, Charles. "Backbone (1)H, (13)C, (15)N NMR assignments of yeast OMP synthase in unliganded form and in complex with orotidine 5'-monophosphate."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
oprtase, polymer, 231 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET B

Entity Sequences (FASTA):
oprtase: PIMLEDYQKNFLELAIECQA LRFGSFKLKSGRESPYFFNL GLFNTGKLLSNLATAYAIAI IQSDLKFDVIFGPAYKGIPL AAIVCVKLAEIGGSKFQNIQ YAFNRKEAKDHGEGGIIVGS ALENKRILIIDDVMTAGTAI NEAFEIISNAKGQVVGSIIA LDRQEVVSTDDKEGLSATQT VSKKYGIPVLSIVSLIHIIT YLEGRITAEEKSKIEQYLQT YGASAENLYFQ

Data sets:
Data typeCount
13C chemical shifts656
15N chemical shifts212
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1omp synthase, 11
2omp synthase, 21

Entities:

Entity 1, omp synthase, 1 231 residues - Formula weight is not available

1   PROILEMETLEUGLUASPTYRGLNLYSASN
2   PHELEUGLULEUALAILEGLUCYSGLNALA
3   LEUARGPHEGLYSERPHELYSLEULYSSER
4   GLYARGGLUSERPROTYRPHEPHEASNLEU
5   GLYLEUPHEASNTHRGLYLYSLEULEUSER
6   ASNLEUALATHRALATYRALAILEALAILE
7   ILEGLNSERASPLEULYSPHEASPVALILE
8   PHEGLYPROALATYRLYSGLYILEPROLEU
9   ALAALAILEVALCYSVALLYSLEUALAGLU
10   ILEGLYGLYSERLYSPHEGLNASNILEGLN
11   TYRALAPHEASNARGLYSGLUALALYSASP
12   HISGLYGLUGLYGLYILEILEVALGLYSER
13   ALALEUGLUASNLYSARGILELEUILEILE
14   ASPASPVALMETTHRALAGLYTHRALAILE
15   ASNGLUALAPHEGLUILEILESERASNALA
16   LYSGLYGLNVALVALGLYSERILEILEALA
17   LEUASPARGGLNGLUVALVALSERTHRASP
18   ASPLYSGLUGLYLEUSERALATHRGLNTHR
19   VALSERLYSLYSTYRGLYILEPROVALLEU
20   SERILEVALSERLEUILEHISILEILETHR
21   TYRLEUGLUGLYARGILETHRALAGLUGLU
22   LYSSERLYSILEGLUGLNTYRLEUGLNTHR
23   TYRGLYALASERALAGLUASNLEUTYRPHE
24   GLN

Samples:

sample_1: oprtase, [U-13C; U-15N; U-2H], 0.9 mM; PIPES 80 mM; DTT 1 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_2
3D HNCAsample_1isotropicsample_conditions_2
3D HN(CO)CAsample_1isotropicsample_conditions_2
3D HN(CA)COsample_1isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_2
3D HN(CO)CACBsample_1isotropicsample_conditions_2

Software:

VNMRJ v2.2D, Varian - collection

TOPSPIN v3.1, Bruker Biospin - collection

NMRPipe v7.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Kazimierczuk - collection, processing

CCPN v2.2, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 18775
PDB
DBJ GAA25343
EMBL CAA32901 CAA46665 CAA89112 CAY81712
GB AAT93179 AHY76362 AJP40607 AJS61787 AJS62213
REF NP_013601
SP P13298
TPG DAA09792
AlphaFold P13298

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts