BMRB Entry 18807

Title:
Chemical shifts for the N-terminal head group of ceSAS-6
Deposition date:
2012-10-25
Original release date:
2019-08-30
Authors:
Erat, Michele; Vakonakis, Ioannis
Citation:

Citation: Hilbert, Manuel; Erat, Michele; Hachet, Virginie; Guichard, Paul; Blank, Iris; Fluckiger, Isabelle; Slater, Leanne; Lowe, Edward; Hatzopoulos, Georgios; Steinmetz, Michel; Gonczy, Pierre; Vakonakis, Ioannis. "Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry"  Proc. Natl. Acad. Sci. U.S.A. 110, 11373-11378 (2013).
PubMed: 23798409

Assembly members:

Assembly members:
ce_SAS-6, polymer, 170 residues, 19425 Da.

Natural source:

Natural source:   Common Name: Nematodes   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified p15b

Data sets:
Data typeCount
13C chemical shifts472
15N chemical shifts153
1H chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ceN SAS-61

Entities:

Entity 1, ceN SAS-6 170 residues - 19425 Da.

GS N-terminal cloning artefact, S123E, I154E

1   GLYSERMETTHRSERLYSILEALALEUPHE
2   ASPGLNTHRLEUILEALASERLEULEUGLN
3   PROLEUSERLEUASNGLNPROASPPHELYS
4   ALATYRLYSTHRLYSVALLYSLEULYSILE
5   SERGLUGLNARGASNGLUTHRSERGLYGLU
6   LYSGLULEULYSPHEGLUILESERARGSER
7   ASPASPPHEGLUPHELEUPHESERGLUTHR
8   LEUASNASNGLULYSTYRGLNILELEUALA
9   ARGASPHISASPLEUTHRVALASPPHEASP
10   ALAPHEPROLYSVALILEILEGLNHISLEU
11   LEUCYSLYSASNILEVALLYSASNLEUGLU
12   GLUASPGLYGLUVALASPALAARGLYSLYS
13   ALAGLYTYRHISGLUILEALAASPPROGLY
14   LYSPROTHRGLUILEASNILEILELEUASP
15   ALAGLULYSASNPHECYSSERPHEGLULEU
16   PHESERLYSTHRPROGLUSERLYSGLYLYS
17   ILEPHESERILELYSLEUHISALAVALARG

Related Database Links:

UNP O62479
PDB PYI
AlphaFold O62479

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks