BMRB Entry 18827

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18827

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Title:
Sequence-specific 1H,13C, and 15N resonance assignment of GATE-16
Deposition date:
2012-11-07
Original release date:
2018-01-12
Authors:
Ma, Peixiang; Hartmann, Rudolf; Matthias, Stoldt; Mohrluder, Jeannine; Schwarten, Melanie; Willbold, Dieter
Citation:

Citation: Ma, Peixiang; Schillinger, Oliver; Schwarten, Melanie; Lecher, Justin; Hartmann, Rudolf; Stoldt, Matthias; Mohrluder, Jeannine; Olubiyi, Olujide; Strodel, Birgit; Willbold, Dieter; Weiergraber, Oliver. "Conformational Polymorphism in Autophagy-Related Protein GATE-16."  Biochemistry 54, 5469-5479 (2015).
PubMed: 26284781

Assembly members:

Assembly members:
GABARAPL2, polymer, 119 residues, 13813.9529 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GABARAPL2: GSMKWMFKEDHSLEHRCVES AKIRAKYPDRVPVIVEKVSG SQIVDIDKRKYLVPSDITVA QFMWIIRKRIQLPSEKAIFL FVDKTVPQSSLTMGQLYEKE KDEDGFLYVAYSGENTFGF

Data sets:
Data typeCount
13C chemical shifts333
15N chemical shifts106
1H chemical shifts208

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Gate161

Entities:

Entity 1, Gate16 119 residues - 13813.9529 Da.

Residues 1-2 represent non-native overhang residues from thrombin cleavage.

1   GLYSERMETLYSTRPMETPHELYSGLUASP
2   HISSERLEUGLUHISARGCYSVALGLUSER
3   ALALYSILEARGALALYSTYRPROASPARG
4   VALPROVALILEVALGLULYSVALSERGLY
5   SERGLNILEVALASPILEASPLYSARGLYS
6   TYRLEUVALPROSERASPILETHRVALALA
7   GLNPHEMETTRPILEILEARGLYSARGILE
8   GLNLEUPROSERGLULYSALAILEPHELEU
9   PHEVALASPLYSTHRVALPROGLNSERSER
10   LEUTHRMETGLYGLNLEUTYRGLULYSGLU
11   LYSASPGLUASPGLYPHELEUTYRVALALA
12   TYRSERGLYGLUASNTHRPHEGLYPHE

Related Database Links:

UniProt P60520
AlphaFold Q9UQF7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks