BMRB Entry 18848
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18848
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Robertson, Ian; Handford, Penny; Redfield, Christina. "Backbone (1)H, (13)C and (15)N resonance assignment of the C-terminal EGF-cbEGF pair of LTBP1 and flanking residues." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23494870
Assembly members:
LTBP1-E3cb15, polymer, 108 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30
Entity Sequences (FASTA):
LTBP1-E3cb15: SASFEELQAEECGILNGCEN
GRCVRVQEGYTCDCFDGYHL
DTAKMTCVDVNECDELNNRM
SLCKNAKCINTDGSYKCLCL
PGYVPSDKPNYCTPLNTALN
LEKDSDLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 373 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LTBP1-E3cb15 | 1 |
| 2 | Calcium ions | 2 |
Entities:
Entity 1, LTBP1-E3cb15 108 residues - Formula weight is not available
| 1 | SER | ALA | SER | PHE | GLU | GLU | LEU | GLN | ALA | GLU | ||||
| 2 | GLU | CYS | GLY | ILE | LEU | ASN | GLY | CYS | GLU | ASN | ||||
| 3 | GLY | ARG | CYS | VAL | ARG | VAL | GLN | GLU | GLY | TYR | ||||
| 4 | THR | CYS | ASP | CYS | PHE | ASP | GLY | TYR | HIS | LEU | ||||
| 5 | ASP | THR | ALA | LYS | MET | THR | CYS | VAL | ASP | VAL | ||||
| 6 | ASN | GLU | CYS | ASP | GLU | LEU | ASN | ASN | ARG | MET | ||||
| 7 | SER | LEU | CYS | LYS | ASN | ALA | LYS | CYS | ILE | ASN | ||||
| 8 | THR | ASP | GLY | SER | TYR | LYS | CYS | LEU | CYS | LEU | ||||
| 9 | PRO | GLY | TYR | VAL | PRO | SER | ASP | LYS | PRO | ASN | ||||
| 10 | TYR | CYS | THR | PRO | LEU | ASN | THR | ALA | LEU | ASN | ||||
| 11 | LEU | GLU | LYS | ASP | SER | ASP | LEU | GLU |
Entity 2, Calcium ions - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: LTBP1-E3cb15, C13- N15, 2 mM; calcium chloride 10 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 30 mM; pH: 5.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
Analysis v2.2.2, CCPN - chemical shift assignment
NMRPipe vJune 2006 Sun Solaris, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 500 MHz
- Home built OMEGA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks