BMRB Entry 18851

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18851

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Title:
HIV-1 Rev ARM peptide (residues T34-R50)
Deposition date:
2012-11-21
Original release date:
2013-09-04
Authors:
Casu, Fabio; Duggan, Brendan; Hennig, Mirko
Citation:

Citation: Casu, Fabio; Duggan, Brendan; Hennig, Mirko. "The Arginine-Rich RNA-Binding Motif of HIV-1 Rev Is Intrinsically Disordered and Folds upon RRE Binding"  Biophys. J. 105, 1004-1017 (2013).
PubMed: 23972852

Assembly members:

Assembly members:
HIV-1_Rev_ARM, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p(H)GB1-derived vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HIV-1_Rev_ARM: GAMATRQARRNRRRRWRERQ RAAAAR

Data sets:
Data typeCount
13C chemical shifts103
15N chemical shifts27
1H chemical shifts163

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 Rev ARM peptide1

Entities:

Entity 1, HIV-1 Rev ARM peptide 26 residues - Formula weight is not available

Residues 1-4 and 22-26 are non-native residues added for expression (TEV-cleavable hexahistidine-GB1 expression tag) and to enhance helical stability.

1   GLYALAMETALATHRARGGLNALAARGARG
2   ASNARGARGARGARGTRPARGGLUARGGLN
3   ARGALAALAALAALAARG

Related Database Links:

BMRB 18852
PDB 2M1A

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