BMRB Entry 18884

Name: CD79b cytosolic domain
Published: 2013-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18884

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CD79b cytosolic domain

Deposition date:

2012-12-07

Original release date:

2013-10-15

Authors:

Mayzel, Maxim

Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" PLoS One 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD79b, polymer, 51 residues, 5702.0248 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Wheat germ - cell freeE. coli - cell free Vector: pEXP5-NT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD79b: SLLDKDDSKAGMEEDHTYEG LDIDQTATYEDIVTLRTGEV KWSVGEHPGQE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	145
15N chemical shifts	48
1H chemical shifts	48

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD79b	1

Entities:

Entity 1, CD79b 51 residues - 5702.0248 Da.

1

SER

LEU

ASP

LYS

ASP

SER

LYS

ALA

2

GLY

MET

GLU

ASP

HIS

THR

TYR

GLU

GLY

3

LEU

ASP

ILE

ASP

GLN

THR

ALA

THR

TYR

GLU

4

ASP

ILE

VAL

THR

LEU

ARG

THR

GLY

GLU

VAL

5

LYS

TRP

SER

VAL

GLY

GLU

HIS

PRO

GLY

GLN

6

GLU

Samples:

Native: CD79b, [U-100% 13C; U-100% 15N], 0.1 mM; DTT 2.0 mM; EDTA 1.0 mM; potassiup phosphate 20.0 mM; azide 0.02%; Complete EDTA-free 1 x; H2O 90%; D2O 10%

1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	Native	isotropic	1
3D HN(CA)CO	Native	isotropic	1
3D HNCA	Native	isotropic	1
3D HNCACB	Native	isotropic	1
3D HNCO	Native	isotropic	1
3D HN(CO)CA	Native	isotropic	1
3D HN(CO)CACB	Native	isotropic	1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - data analysis

MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing

TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

Varian UnityInova 600 MHz

UNP	P40259
BMRB	19650 19651
DBJ	BAA25652 BAD96674 BAD96930 BAG36353
EMBL	CAA58522
GB	AAA58387 AAA64459 AAA72424 AAB24822 AAC60654
REF	NP_000617 NP_001035022 NP_001230841 NP_001248454 NP_067613
SP	P40259
TPG	DAA18410
AlphaFold	Q9BU06 P40259