BMRB Entry 18885
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18885
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Title: S. cerevisiae proteasome regulatory particle ATPase Rpt6 C-terminal domain PubMed: 23562395
Deposition date: 2012-12-07 Original release date: 2013-02-15
Authors: Ehlinger, Aaron; Walters, Kylie
Citation: Ehlinger, Aaron; Park, Soyeon; Fahmy, Amr; Lary, Jeffrey; Cole, James; Finley, Daniel; Walters, Kylie. "Conformational dynamics of the rpt6 ATPase in proteasome assembly and rpn14 binding." Structure 21, 753-765 (2013).
Assembly members:
Rpt6, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSF-duet1
Entity Sequences (FASTA):
Rpt6: MHHHHHHSQHMPPSVAARAE
ILRIHSRKMNLTRGINLRKV
AEKMNGCSGADVKGVCTEAG
MYALRERRIHVTQEDFELAV
GKVMNKNQETAISVAKLFK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rpt6 4-helix bundle | 1 |
| 2 | Rpt6 partially unfolded | 1 |
Entities:
Entity 1, Rpt6 4-helix bundle 99 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | GLN | HIS | ||||
| 2 | MET | PRO | PRO | SER | VAL | ALA | ALA | ARG | ALA | GLU | ||||
| 3 | ILE | LEU | ARG | ILE | HIS | SER | ARG | LYS | MET | ASN | ||||
| 4 | LEU | THR | ARG | GLY | ILE | ASN | LEU | ARG | LYS | VAL | ||||
| 5 | ALA | GLU | LYS | MET | ASN | GLY | CYS | SER | GLY | ALA | ||||
| 6 | ASP | VAL | LYS | GLY | VAL | CYS | THR | GLU | ALA | GLY | ||||
| 7 | MET | TYR | ALA | LEU | ARG | GLU | ARG | ARG | ILE | HIS | ||||
| 8 | VAL | THR | GLN | GLU | ASP | PHE | GLU | LEU | ALA | VAL | ||||
| 9 | GLY | LYS | VAL | MET | ASN | LYS | ASN | GLN | GLU | THR | ||||
| 10 | ALA | ILE | SER | VAL | ALA | LYS | LEU | PHE | LYS |
Samples:
sample_1: Rpt6, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 mM; beta-mercaptoethanol 14 mM; sodium chloride 60 mM; sodium phosphate 20 mM; H20 90%; D20 10%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 2D ZZ-Exchange Spectroscopy | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | GAA23341 |
| EMBL | CAA47023 CAA96750 CAY79712 CCD24599 |
| GB | AAA35138 AAB35417 AAT93154 AHY79325 AJP38743 |
| PRF | 1813279A |
| REF | NP_011467 XP_003669842 |
| SP | Q01939 |
| TPG | DAA08053 |
| AlphaFold | Q01939 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts