BMRB Entry 18930

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18930

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Title:
Solution NMR assignments of the phosphorylation-mimicking mutant of V5 domain from Protein Kinase C alpha, in complex with DPC micelles
Deposition date:
2013-01-02
Original release date:
2014-02-14
Authors:
Yang, Yuan; Igumenova, Tatyana
Citation:

Citation: Yang, Yuan; Igumenova, Tatyana. "The C-terminal V5 domain of Protein Kinase C is intrinsically disordered, with propensity to associate with a membrane mimetic."  PLoS ONE 8, e65699-e65699 (2013).
PubMed: 23762412

Assembly members:

Assembly members:
T638E/S657E_V5_domain,_residue_606-672, polymer, 68 residues, 7666.6 Da.
dodecyl 2-(trimethylammonio)ethyl phosphate, non-polymer, 351.462 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET31b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T638E/S657E_V5_domain,_residue_606-672: ENREIQPPFKPKVSGKGAEN FDKFFTRGQPVLEPPDQLVI ANIDQSDFEGFEYVNPQFVH PILQSAVX

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts52
1H chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1T638E/S657E V5 domain of Protein Kinase C alpha1
2DPV2

Entities:

Entity 1, T638E/S657E V5 domain of Protein Kinase C alpha 68 residues - 7666.6 Da.

Residue 673 (HSL) is a non-standard Homoserine-lactone that resulted from CNBr cleavage at Methionine residue

1   GLUASNARGGLUILEGLNPROPROPHELYS
2   PROLYSVALSERGLYLYSGLYALAGLUASN
3   PHEASPLYSPHEPHETHRARGGLYGLNPRO
4   VALLEUGLUPROPROASPGLNLEUVALILE
5   ALAASNILEASPGLNSERASPPHEGLUGLY
6   PHEGLUTYRVALASNPROGLNPHEVALHIS
7   PROILELEUGLNSERALAVALHSL

Entity 2, DPV - C17 H38 N O4 P - 351.462 Da.

1   DPV

Related Database Links:

BMRB 18927 18928 18929
PDB 4RA4

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks