BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18981

Title: DNA containing a cluster of 8-oxo-guanine and THF lesion   PubMed: 24384094

Deposition date: 2013-01-28 Original release date: 2014-01-06

Authors: Zalesak, Jan; Jourdan, Muriel; Constant, Jean-Francois; Lourdin, Morgane

Citation: Zaleak, Jan; Lourdin, Morgane; Krej, Lumr; Constant, Jean-Francois; Jourdan, Muriel. "Structure and dynamics of DNA duplexes containing a cluster of mutagenic 8-oxoguanine and abasic site lesions."  J. Mol. Biol. 426, 1524-1538 (2014).

Assembly members:
DNA_A_strand, polymer, 11 residues, 3146.065 Da.
DNA_B_strand, polymer, 11 residues, 3471.274 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_A_strand: CGCTCXCACGC
DNA_B_strand: GCGTGGGAXCG

Data sets:
Data typeCount
1H chemical shifts181
31P chemical shifts21

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA_A_strand1
2DNA_B_strand2

Entities:

Entity 1, DNA_A_strand 11 residues - 3146.065 Da.

1   DCDGDCDTDC3DRDCDADCDG
2   DC

Entity 2, DNA_B_strand 11 residues - 3471.274 Da.

1   DGDCDGDTDGDGDGDA8OGDC
2   DG

Samples:

sample_1: DNA_A_strand 0.97 mM; DNA_B_strand 0.97 mM; D2O 100%

sample_conditions_1: ionic strength: 120 mM; pH: 6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz