BMRB Entry 18992

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18992

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Title:
Backbone resonance assignment of [2Fe-2S]-ferredoxin in its reduced state
Deposition date:
2013-01-30
Original release date:
2014-02-14
Authors:
Kim, Jin Hae; Markley, John
Citation:

Citation: Kim, Jin Hae; Frederick, Ronnie; Reinen, Nichole; Troupis, Andrew; Markley, John. "[2Fe-2S]-Ferredoxin Binds Directly to Cysteine Desulfurase and Supplies an Electron for Iron-Sulfur Cluster Assembly but Is Displaced by the Scaffold Protein or Bacterial Frataxin."  J. Am. Chem. Soc. 135, 8117-8120 (2013).
PubMed: 23682711

Assembly members:

Assembly members:
ferredoxin, polymer, 111 residues, Formula weight is not available
entity_FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST42

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ferredoxin: MPKIVILPHQDLCPDGAVLE ANSGETILDAALRNGIEIEH ACEKSCACTTCHCIVREGFD SLPESSEQEDDMLDKAWGLE PESRLSCQARVTDEDLVVEI PRYTINHAREH

Data sets:
Data typeCount
13C chemical shifts174
15N chemical shifts79
1H chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ferredoxin1
22Fe-2S cluster2

Entities:

Entity 1, ferredoxin 111 residues - Formula weight is not available

1   METPROLYSILEVALILELEUPROHISGLN
2   ASPLEUCYSPROASPGLYALAVALLEUGLU
3   ALAASNSERGLYGLUTHRILELEUASPALA
4   ALALEUARGASNGLYILEGLUILEGLUHIS
5   ALACYSGLULYSSERCYSALACYSTHRTHR
6   CYSHISCYSILEVALARGGLUGLYPHEASP
7   SERLEUPROGLUSERSERGLUGLNGLUASP
8   ASPMETLEUASPLYSALATRPGLYLEUGLU
9   PROGLUSERARGLEUSERCYSGLNALAARG
10   VALTHRASPGLUASPLEUVALVALGLUILE
11   PROARGTYRTHRILEASNHISALAARGGLU
12   HIS

Entity 2, 2Fe-2S cluster - Fe2 S2 - 175.820 Da.

1   FES

Related Database Links:

BMRB 18991 19273
PDB 1I7H
DBJ BAA16415 BAB36814 BAG78335 BAI26770 BAI31799
EMBL CAP76977 CAQ32898 CAQ88191 CAQ99416 CAR03967
GB AAA23755 AAC75578 AAG57639 AAN44071 AAN81500
REF NP_311418 NP_417020 NP_708364 WP_001124467 WP_001124468
SP P0A9R4 P0A9R5 P0A9R6
AlphaFold P0A9R4 P0A9R5 P0A9R6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks