BMRB Entry 19064
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19064
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Title: TIAR RRM2 chemical shifts in bound to RNA 5'-UUAUUU-3' PubMed: 23603827
Deposition date: 2013-02-28 Original release date: 2014-02-13
Authors: Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Miriam; Wilce, Matthew; Wilce, Jacqueline
Citation: Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Myriam; Wilce, Matthew; Wilce, Jacqueline. "Distinct binding properties of TIAR RRMs and linker region." RNA Biol 10, 579-589 (2013).
Assembly members:
TIAR_RRM2, polymer, 101 residues, Formula weight is not available
RNA_TIAR_RRM2, polymer, 6 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
TIAR_RRM2: GSPGISGGGGGIPSNHFHVF
VGDLSPEITTEDIKSAFAPF
GKISDARVVKDMATGKSKGY
GFVSFYNKLDAENAIVHMGG
QWLGGRQIRTNWATRKPPAP
K
RNA_TIAR_RRM2: UUAUUU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 246 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TIAR RRM2 | 1 |
| 2 | RNA 5'-UUAUUU-3' | 2 |
Entities:
Entity 1, TIAR RRM2 101 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLY | ILE | SER | GLY | GLY | GLY | GLY | ||||
| 2 | GLY | ILE | PRO | SER | ASN | HIS | PHE | HIS | VAL | PHE | ||||
| 3 | VAL | GLY | ASP | LEU | SER | PRO | GLU | ILE | THR | THR | ||||
| 4 | GLU | ASP | ILE | LYS | SER | ALA | PHE | ALA | PRO | PHE | ||||
| 5 | GLY | LYS | ILE | SER | ASP | ALA | ARG | VAL | VAL | LYS | ||||
| 6 | ASP | MET | ALA | THR | GLY | LYS | SER | LYS | GLY | TYR | ||||
| 7 | GLY | PHE | VAL | SER | PHE | TYR | ASN | LYS | LEU | ASP | ||||
| 8 | ALA | GLU | ASN | ALA | ILE | VAL | HIS | MET | GLY | GLY | ||||
| 9 | GLN | TRP | LEU | GLY | GLY | ARG | GLN | ILE | ARG | THR | ||||
| 10 | ASN | TRP | ALA | THR | ARG | LYS | PRO | PRO | ALA | PRO | ||||
| 11 | LYS |
Entity 2, RNA 5'-UUAUUU-3' 6 residues - Formula weight is not available
| 1 | U | U | A | U | U | U |
Samples:
TIAR_RRM2_apo1: entity_1, [U-13C; U-15N], mM; entity_2 mM; D2O 10%; H2O 90%
TIAR_RRM2_apo2: entity_1, [U-13C; U-15N], mM; entity_2 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | TIAR_RRM2_apo1 | isotropic | sample_conditions_1 |
| 3D HNCACB | TIAR_RRM2_apo1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | TIAR_RRM2_apo1 | isotropic | sample_conditions_1 |
| 3D HNCO | TIAR_RRM2_apo1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts