BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19180

Title: Trp-cage Circular Permutant   PubMed: 24376912

Deposition date: 2013-04-18 Original release date: 2013-12-23

Authors: Byrne, Aimee; Andersen, Niels; Kier, Brandon

Citation: Byrne, Aimee; Kier, Brandon; Williams, D.; Scian, Michele; Andersen, Niels. "Circular Permutation of the Trp-cage: Fold Rescue upon Addition of a Hydrophobic Staple."  RSC Adv. 2013, .-. (2013).

Assembly members:
Trp-cage, polymer, 21 residues, 2216.412 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not applicable

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
Trp-cage: RPPPSDXAAYAQWLADXGWA S

Data sets:
Data typeCount
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Trp-cage Circular Permutant1

Entities:

Entity 1, Trp-cage Circular Permutant 21 residues - 2216.412 Da.

1   ARGPROPROPROSERASPAIBALAALATYR
2   ALAGLNTRPLEUALAASPDALGLYTRPALA
3   SER

Samples:

sample_1: sodium phosphate 50 mM; H2O 90%; D2O 10%; Trp-cage 1.5 mM

sample_conditions_1: ionic strength: 0.11 M; pH: 7; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DMX 800 MHz

Related Database Links:

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