BMRB Entry 19217
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19217
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Jia, Xinying; Schulte, Leigh; Loukas, Alex; Pickering, Darren; Pearson, Mark; Mobli, Mehdi; Jones, Alun; Rosengren, Karl; Daly, Norelle; Gobert, Geoffrey; Jones, Malcolm; Craik, David; Mulvenna, Jason. "Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni." J. Biol. Chem. 289, 7151-7163 (2014).
PubMed: 24429291
Assembly members:
SmTSP2EC2, polymer, 81 residues, 9091.423 Da.
Natural source: Common Name: Flatworm Taxonomy ID: 6183 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Schistosoma mansoni
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLICMBP
Entity Sequences (FASTA):
SmTSP2EC2: GSNEKPKVKKHITSALKKLV
DKYRNDEHVRKVFDEIQQKL
HCCGADSPKDYGENPPTSCS
KDGVQFTEGCIKKVSDLSKA
H
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 274 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 556 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SmTSP2EC2 | 1 |
Entities:
Entity 1, SmTSP2EC2 81 residues - 9091.423 Da.
| 1 | GLY | SER | ASN | GLU | LYS | PRO | LYS | VAL | LYS | LYS | ||||
| 2 | HIS | ILE | THR | SER | ALA | LEU | LYS | LYS | LEU | VAL | ||||
| 3 | ASP | LYS | TYR | ARG | ASN | ASP | GLU | HIS | VAL | ARG | ||||
| 4 | LYS | VAL | PHE | ASP | GLU | ILE | GLN | GLN | LYS | LEU | ||||
| 5 | HIS | CYS | CYS | GLY | ALA | ASP | SER | PRO | LYS | ASP | ||||
| 6 | TYR | GLY | GLU | ASN | PRO | PRO | THR | SER | CYS | SER | ||||
| 7 | LYS | ASP | GLY | VAL | GLN | PHE | THR | GLU | GLY | CYS | ||||
| 8 | ILE | LYS | LYS | VAL | SER | ASP | LEU | SER | LYS | ALA | ||||
| 9 | HIS |
Samples:
sample_1: SmTSP2EC2, [U-100% 13C; U-100% 15N], 1.4 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 4D HCC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D (H)CB-(CGCCTOCSY)-Har | sample_1 | isotropic | sample_conditions_1 |
| Tyr-selective 2D (H)CB-(CGCCTOCSY)-Har | sample_1 | isotropic | sample_conditions_1 |
| Phe-selective 2D (H)CB-(CGCCTOCSY)-Har | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Bruker Biospin, CCPN, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, collection, peak picking, processing, structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks