BMRB Entry 19308
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19308
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Title: Biochemical effect of S-67 phosphorylation on UVI31+ from C. reinhardtii PubMed: 23979961
Deposition date: 2013-06-18 Original release date: 2014-02-13
Authors: Singh, Himanshu; Verma, Deepshikha; Chary, KVR
Citation: Singh, Himanshu; Rao, B.; Chary, Kandala. "(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
Phophorylated_UVI31+, polymer, 123 residues, 13360 Da.
Natural source: Common Name: Chlamydomonas reinhardtii Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET and pQE-30
Entity Sequences (FASTA):
Phophorylated_UVI31+: MRGSHHHHHHGSHVISSIAS
RGSMAEHQLGPIAGAIKSKV
EAALSPTHFKLINDSHKHAG
HYARDGSTASDAGETHFRLE
VTSDAFKGLTLVKRHQLIYG
LLSDEFKAGLHALSMTTKTP
AEQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UVI31+ | 1 |
Entities:
Entity 1, UVI31+ 123 residues - 13360 Da.
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | HIS | VAL | ILE | SER | SER | ILE | ALA | SER | ||||
| 3 | ARG | GLY | SER | MET | ALA | GLU | HIS | GLN | LEU | GLY | ||||
| 4 | PRO | ILE | ALA | GLY | ALA | ILE | LYS | SER | LYS | VAL | ||||
| 5 | GLU | ALA | ALA | LEU | SER | PRO | THR | HIS | PHE | LYS | ||||
| 6 | LEU | ILE | ASN | ASP | SER | HIS | LYS | HIS | ALA | GLY | ||||
| 7 | HIS | TYR | ALA | ARG | ASP | GLY | SER | THR | ALA | SER | ||||
| 8 | ASP | ALA | GLY | GLU | THR | HIS | PHE | ARG | LEU | GLU | ||||
| 9 | VAL | THR | SER | ASP | ALA | PHE | LYS | GLY | LEU | THR | ||||
| 10 | LEU | VAL | LYS | ARG | HIS | GLN | LEU | ILE | TYR | GLY | ||||
| 11 | LEU | LEU | SER | ASP | GLU | PHE | LYS | ALA | GLY | LEU | ||||
| 12 | HIS | ALA | LEU | SER | MET | THR | THR | LYS | THR | PRO | ||||
| 13 | ALA | GLU | GLN |
Samples:
sample_1: Phophorylated UVI31+, [U-99% 13C; U-99% 15N], 0.5 mM; phosphate buffer 50 mM; ATP 1.5 mM; MgCl2 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2, bruker - chemical shift assignment, processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts