BMRB Entry 19320
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19320
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Title: 1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15
Deposition date: 2013-06-25 Original release date: 2015-03-23
Authors: Kashyap, Maruthi; Ganguly, Akshay; Bhavesh, Neel Sarovar
Citation: Kashyap, Maruthi; Ganguly, Akshay; Bhavesh, Neel Sarovar. "1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15" .
Assembly members:
Taf15, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Taf15: GSHMSDNNTIFVQGLGEGVS
TDQVGEFFKQIGIIKTNKKT
GKPMINLYTDKDTGKPKGEA
TVSFDDPPSAKAAIDWFDGK
EFHGNIIKVSFATRRPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 412 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 633 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Taf15 | 1 |
Entities:
Entity 1, Taf15 97 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | SER | ASP | ASN | ASN | THR | ILE | ||||
| 2 | PHE | VAL | GLN | GLY | LEU | GLY | GLU | GLY | VAL | SER | ||||
| 3 | THR | ASP | GLN | VAL | GLY | GLU | PHE | PHE | LYS | GLN | ||||
| 4 | ILE | GLY | ILE | ILE | LYS | THR | ASN | LYS | LYS | THR | ||||
| 5 | GLY | LYS | PRO | MET | ILE | ASN | LEU | TYR | THR | ASP | ||||
| 6 | LYS | ASP | THR | GLY | LYS | PRO | LYS | GLY | GLU | ALA | ||||
| 7 | THR | VAL | SER | PHE | ASP | ASP | PRO | PRO | SER | ALA | ||||
| 8 | LYS | ALA | ALA | ILE | ASP | TRP | PHE | ASP | GLY | LYS | ||||
| 9 | GLU | PHE | HIS | GLY | ASN | ILE | ILE | LYS | VAL | SER | ||||
| 10 | PHE | ALA | THR | ARG | ARG | PRO | GLU |
Samples:
sample_1: Taf15, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 700 MHz
Related Database Links:
| BMRB | 19561 |
| PDB | |
| DBJ | BAA33811 BAA33812 BAB46889 BAC34676 BAE27086 |
| EMBL | CAA67398 |
| GB | AAC50932 AAH46099 AAI37592 AAI66769 AAO13485 |
| REF | NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961 |
| SP | Q92804 |
| TPG | DAA19087 |
| AlphaFold | Q92804 |
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