BMRB Entry 19370
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19370
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Title: calbindin D9k(P47M+C80) calcium bound form PubMed: 23933251
Deposition date: 2013-07-16 Original release date: 2014-02-13
Authors: Hembram, Dambarudhar; Haremaki, Takahiro; Hamatsu, Jumpei; Inoue, Jin; Kamoshida, Hajime; Ikeya, Teppei; Mishima, Masaki; Mikawa, Tsutomu; Hayashi, Nobuhiro; Shirakawa, Masahiro; Ito, Yutaka
Citation: Hembram, Dambarudhar Shiba Sankar; Haremaki, Takahiro; Hamatsu, Jumpei; Inoue, Jin; Kamoshida, Hajime; Ikeya, Teppei; Mishima, Masaki; Mikawa, Tsutomu; Hayashi, Nobuhiro; Shirakawa, Masahiro; Ito, Yutaka. "An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca2+ concentration in HeLa cells." Biochem. Biophys. Res. Commun. 438, 653-659 (2013).
Assembly members:
calbindin_D9k_calcium_bound-form, polymer, 77 residues, 40.078 Da.
CALCIUM ION, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
calbindin_D9k_calcium_bound-form: KKSPEELKRIFEKYAAKEGD
PDQLSKDELKLLIQAEFPSL
LKGMNTLDDLFQELDKNGDG
EVSFEEFQVLVKKISQC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 218 |
15N chemical shifts | 69 |
1H chemical shifts | 69 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | calbindin D9k | 1 |
2 | calcium ion | 2 |
Entities:
Entity 1, calbindin D9k 77 residues - 40.078 Da.
1 | LYS | LYS | SER | PRO | GLU | GLU | LEU | LYS | ARG | ILE | ||||
2 | PHE | GLU | LYS | TYR | ALA | ALA | LYS | GLU | GLY | ASP | ||||
3 | PRO | ASP | GLN | LEU | SER | LYS | ASP | GLU | LEU | LYS | ||||
4 | LEU | LEU | ILE | GLN | ALA | GLU | PHE | PRO | SER | LEU | ||||
5 | LEU | LYS | GLY | MET | ASN | THR | LEU | ASP | ASP | LEU | ||||
6 | PHE | GLN | GLU | LEU | ASP | LYS | ASN | GLY | ASP | GLY | ||||
7 | GLU | VAL | SER | PHE | GLU | GLU | PHE | GLN | VAL | LEU | ||||
8 | VAL | LYS | LYS | ILE | SER | GLN | CYS |
Entity 2, calcium ion - Ca - 40.078 Da.
1 | CA |
Samples:
sample: calbindin D9k calcium bound-form, [U-100% 13C; U-100% 15N], 1.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.15 mM; pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample | isotropic | sample_conditions_1 |
3D HNCO | sample | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample | isotropic | sample_conditions_1 |
3D CBCANH | sample | isotropic | sample_conditions_1 |
Software:
AZARA v2.8, Boucher - processing
QME, Daniel O'Donovan and Ernest Laue - processing
ANALYSIS v2.2.2, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
BMRB | 19369 19371 |
DBJ | BAI46392 |
EMBL | CAA46699 |
GB | AAA35637 AAA35638 AAI11918 AAI12175 EAW98916 |
REF | NP_004048 XP_001102900 XP_001139782 XP_002831466 XP_003261140 |
SP | P29377 |
AlphaFold | P29377 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts