BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19393

Title: Solid-state NMR sequential assignment of Osaka-Mutant Amyloid-beta (A 1-40 E22 ) fibrils   PubMed: 24395155

Deposition date: 2013-07-29 Original release date: 2014-02-13

Authors: Huber, Matthias; Ovchinnikova, Oxana; Schuetz, Anne; Glockshuber, Rudi; Meier, Beat; Bockmann, Anja

Citation: Huber, Matthias; Ovchinnikova, Oxana Yu; Schutz, Anne; Glockshuber, Rudi; Meier, Beat; Bockmann, Anja. "Solid-state NMR sequential assignment of Osaka-mutant amyloid-beta (A1-40 E22) fibrils."  Biomol. NMR Assignments ., .-. (2014).

Assembly members:
Abeta, polymer, 39 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETA

Entity Sequences (FASTA):
Abeta: DAEFRHDSGYEVHHQKLVFF ADVGSNKGAIIGLMVGGVV

Data typeCount
13C chemical shifts413
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta1

Entities:

Entity 1, Abeta 39 residues - Formula weight is not available

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAASPVALGLYSERASNLYSGLYALAILE
4   ILEGLYLEUMETVALGLYGLYVALVAL

Samples:

UL: Abeta, [U-100% 13C; U-100% 15N], 40 ± 20 w/v; H2O 60 ± 20 w/v

U13C: Abeta, [U-100% 13C], 40 ± 20 w/v; H2O 60 ± 20 w/v

sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBULsolidsample_conditions_1
2D 1H-1H NOESYU13Csolidsample_conditions_1

Software:

CcpNMR v2.2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11435 15775 17159 17186 17764 17793 17794 17795 17796 18052 18127 18128 18129 18131 19009 19309 25218 25289 25429 26508 26516
PDB
DBJ BAA22264 BAA84580 BAD51938 BAE01907 BAG10647
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GB AAA35540 AAA36829 AAA51722 AAA51726 AAA51727
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014
SP P05067 P53601 P79307 Q28053 Q28280
TPG DAA33655
AlphaFold Q28053 P05067 P53601 P79307 Q28280