BMRB Entry 19419
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19419
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Title: 1H, 13C, and 15N Chemical Shift Assignments of C-ala domain from Bizionia argentinensis PubMed: 24258519
Deposition date: 2013-08-09 Original release date: 2014-02-13
Authors: Smal, Clara; Zanzoni, Serena; D'Onofrio, Mariapina; Assfalg, Michael; Cicero, Daniel; Molinari, Henriette
Citation: Smal, Clara; Zanzoni, Serena; D'Onofrio, Mariapina; Molinari, Henriette; Cicero, Daniel; Assfalg, Michael. "(1)H, (15)N and (13)C chemical shift assignments of the C-Ala domain of the alanyl-tRNA synthetase of the psychrophilic bacterium Bizionia argentinensis sp. nov." Biomol NMR Assign 8, 415-418 (2014).
Assembly members:
C-ala_domain, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: Bizionia argentinensis Taxonomy ID: 456455 Superkingdom: Bacteria Kingdom: not available Genus/species: Bizionia argentinensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST-527
Entity Sequences (FASTA):
C-ala_domain: MKAKNLKGELKNEITEINGI
HFLAKKVDLDAGGIKDLCFE
LGSQYDNLFLLFGAENDGKA
LLSCYVSKELVERKGLNAGQ
IVRELGKFIQGGGGGQPFFA
TAGGKNPAGIAEALEAAKKY
LV
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 530 |
15N chemical shifts | 130 |
1H chemical shifts | 860 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | C-Ala domain | 1 |
Entities:
Entity 1, C-Ala domain 122 residues - Formula weight is not available
1 | MET | LYS | ALA | LYS | ASN | LEU | LYS | GLY | GLU | LEU | ||||
2 | LYS | ASN | GLU | ILE | THR | GLU | ILE | ASN | GLY | ILE | ||||
3 | HIS | PHE | LEU | ALA | LYS | LYS | VAL | ASP | LEU | ASP | ||||
4 | ALA | GLY | GLY | ILE | LYS | ASP | LEU | CYS | PHE | GLU | ||||
5 | LEU | GLY | SER | GLN | TYR | ASP | ASN | LEU | PHE | LEU | ||||
6 | LEU | PHE | GLY | ALA | GLU | ASN | ASP | GLY | LYS | ALA | ||||
7 | LEU | LEU | SER | CYS | TYR | VAL | SER | LYS | GLU | LEU | ||||
8 | VAL | GLU | ARG | LYS | GLY | LEU | ASN | ALA | GLY | GLN | ||||
9 | ILE | VAL | ARG | GLU | LEU | GLY | LYS | PHE | ILE | GLN | ||||
10 | GLY | GLY | GLY | GLY | GLY | GLN | PRO | PHE | PHE | ALA | ||||
11 | THR | ALA | GLY | GLY | LYS | ASN | PRO | ALA | GLY | ILE | ||||
12 | ALA | GLU | ALA | LEU | GLU | ALA | ALA | LYS | LYS | TYR | ||||
13 | LEU | VAL |
Samples:
sample_1: C-ala domain, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 100 mM
sample_2: C-ala domain, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 100 mM
sample_3: C-ala domain, [U-99% 15N], 0.5 mM; sodium phosphate 100 mM
sample_4: C-ala domain 0.5 mM; sodium phosphate 100 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_4 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_3 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts