BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19437

Title: Membrane induced structure of the mammalian tachykinin neuropeptide gamma   PubMed: 15522780

Deposition date: 2013-08-19 Original release date: 2013-09-23

Authors: Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha

Citation: Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha. "Membrane-induced structure of the mammalian tachykinin neuropeptide gamma"  J. Struct. Biol. 148, 315-325 (2004).

Assembly members:
NPgamma, polymer, 21 residues, 4637.265 Da.
5-ACETYLAMINO-4-AMINO-6-(PHENETHYL-PROPYL-CARBAMOYL)-5,6-DIHYDRO-4H-PYRAN-2-CARBOXYLIC ACID, non-polymer, 389.445 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
NPgamma: DAGHGQISHKRHKTDSFVGL M

Data sets:
Data typeCount
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mammalian tachykinin neuropeptide gamma1
2DPC2

Entities:

Entity 1, mammalian tachykinin neuropeptide gamma 21 residues - 4637.265 Da.

1   ASPALAGLYHISGLYGLNILESERHISLYS
2   ARGHISLYSTHRASPSERPHEVALGLYLEU
3   MET

Entity 2, DPC - C20 H27 N3 O5 - 389.445 Da.

1   DPC

Samples:

sample_1: entity 4 mg; DPC, [U-100% 2H], 250 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 2.4; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - data analysis, processing

DIANA, Guntert, Braun and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz