BMRB Entry 19467
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19467
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Title: 10 C-terminal PKCalpha residues PubMed: 25023278
Deposition date: 2013-08-29 Original release date: 2019-12-06
Authors: Erlendsson, Simon; Teilum, Kaare
Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand" J. Biol. Chem. 289, 25327-25340 (2014).
Assembly members:
PKCalpha_C-term, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
PKCalpha_C-term: PQFVHPILQSAV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PKCalpha C-term | 1 |
Entities:
Entity 1, PKCalpha C-term 12 residues - Formula weight is not available
| 1 | PRO | GLN | PHE | VAL | HIS | PRO | ILE | LEU | GLN | SER | ||||
| 2 | ALA | VAL |
Samples:
sample_1: PKCalpha C-term 4 mM; PICK1 50 uM; D2O 10%; DTT 0.25 mM; TRIS 50 mM; Triton X 100 0.01%; sodium cloride 125 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.4; pressure: ambient atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPN_analysis, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian INOVA 800 MHz