BMRB Entry 19518

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19518

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Title:
Solution NMR assignment of the heavy chain complex of the human cardiac myosin regulatory light chain
Deposition date:
2013-09-23
Original release date:
2014-01-29
Authors:
Rostkova, Elena; Gautel, Mathias; Pfuhl, Mark
Citation:

Citation: Rostkova, Elena; Gautel, Mathias; Pfuhl, Mark. "Solution NMR assignment of the heavy chain complex of the human cardiac myosin regulatory light chain"  J. Biomol. NMR 9, 51-53 (2014).
PubMed: 24414277

Assembly members:

Assembly members:
RLC, polymer, 166 residues, Formula weight is not available
HC, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet8c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RLC: MAPKKAKKRAGGANSNVFSM FEQTQIQEFKEAFTIMDQNR DGFIDKNDLRDTFAALGRVN VKNEEIDEMIKEAPGPINFT VFLTMFGEKLKGADPEETIL NAFKVFDPEGKGVLKADYVR EMLTTQAERFSKEEVDQMFA AFPPDVTGNLDYKNLVHIIT HGEEKD
HC: ERRDSLLVIQWNIRAFMGVK NWPWMKLYFKIK

Data sets:
Data typeCount
13C chemical shifts788
15N chemical shifts203
1H chemical shifts1347

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1light chain1
2heavy chain2

Entities:

Entity 1, light chain 166 residues - Formula weight is not available

1   METALAPROLYSLYSALALYSLYSARGALA
2   GLYGLYALAASNSERASNVALPHESERMET
3   PHEGLUGLNTHRGLNILEGLNGLUPHELYS
4   GLUALAPHETHRILEMETASPGLNASNARG
5   ASPGLYPHEILEASPLYSASNASPLEUARG
6   ASPTHRPHEALAALALEUGLYARGVALASN
7   VALLYSASNGLUGLUILEASPGLUMETILE
8   LYSGLUALAPROGLYPROILEASNPHETHR
9   VALPHELEUTHRMETPHEGLYGLULYSLEU
10   LYSGLYALAASPPROGLUGLUTHRILELEU
11   ASNALAPHELYSVALPHEASPPROGLUGLY
12   LYSGLYVALLEULYSALAASPTYRVALARG
13   GLUMETLEUTHRTHRGLNALAGLUARGPHE
14   SERLYSGLUGLUVALASPGLNMETPHEALA
15   ALAPHEPROPROASPVALTHRGLYASNLEU
16   ASPTYRLYSASNLEUVALHISILEILETHR
17   HISGLYGLUGLULYSASP

Entity 2, heavy chain 32 residues - Formula weight is not available

1   GLUARGARGASPSERLEULEUVALILEGLN
2   TRPASNILEARGALAPHEMETGLYVALLYS
3   ASNTRPPROTRPMETLYSLEUTYRPHELYS
4   ILELYS

Samples:

N: RLC, [U-95% 15N], 0.25 ± 0.01 mM; HC, [U-95% 15N], 0.25 ± 0.01 mM; HEPES 20 mM; Arginine 50 mM; Glutamic acid 50 mM; DTT 2 mM; NaN3 0.02%

NC: RLC, [U-95% 13C; U-95% 15N], 0.25 ± 0.01 mM; HC, [U-95% 13C; U-95% 15N], 0.25 ± 0.01 mM; HEPES 20 mM; Arginine 50 mM; Glutamic acid 50 mM; DTT 2 mM; NaN3 0.02%

ND: RLC, [U-95% 15N; U-85% 2H], 0.25 ± 0.01 mM; HC, [U-95% 15N; U-85% 2H]], 0.25 ± 0.01 mM; HEPES 20 mM; Arginine 50 mM; Glutamic acid 50 mM; DTT 2 mM; NaN3 0.02%

NCD: RLC, [U-95% 13C; U-90% 15N; U-85% 2H], 0.25 ± 0.01 mM; HC, [U-95% 13C; U-95% 15N; U-85% 2H], 0.25 ± 0.01 mM; HEPES 20 mM; Arginine 50 mM; Glutamic acid 50 mM; DTT 2 mM; NaN3 0.02%

NC_D2O: RLC, [U-95% 13C; U-95% 15N], 0.25 ± 0.01 mM; HC, [U-95% 13C; U-95% 15N], 0.25 ± 0.01 mM; HEPES 20 mM; Arginine 50 mM; Glutamic acid 50 mM; DTT 2 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNisotropicsample_conditions_1
3D 1H-15N NOESYNisotropicsample_conditions_1
3D HNCACBNCDisotropicsample_conditions_1
3D CBCA(CO)NHNCDisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticNC_D2Oisotropicsample_conditions_1
3D 1H-15N NOESYNDisotropicsample_conditions_1
3D 1H-13C NOESY aromaticNC_D2Oisotropicsample_conditions_1
3D HNCONCisotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

CCPN_Analysis v2.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

UNP P10916 P12883
AlphaFold Q16123 Q9UMM8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks