BMRB Entry 19579
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PDB ID: 2mfz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19579
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Otikovs, Martins; Jaudzems, Kristaps; Andersson, Marlene; Chen, Gefei; Landreh, Michael; Nordling, Kerstin; Kronqvist, Nina; Westermark, Per; Jornvall, Hans; Knight, Stefan; Ridderstrale, Yvonne; Holm, Lena; Meng, Qing; Chesler, Mitchell; Johansson, Jan; Rising, Anna. "Carbonic Anhydrase Generates CO2 and H+ That Drive Spider Silk Formation Via Opposite Effects on the Terminal Domains" Plos Biol. 12, e1001921-e1001921 (2014).
PubMed: 25093327
Assembly members:
MiSp-CT, polymer, 125 residues, 11766.900 Da.
Natural source: Common Name: Orbweaver spider Taxonomy ID: 182803 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Araneus ventricosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET vector
Entity Sequences (FASTA):
MiSp-CT: GSGNSVTSGGYGYGTSAAAG
AGVAAGSYAGAVNRLSSAEA
ASRVSSNIAAIASGGASALP
SVISNIYSGVVASGVSSNEA
LIQALLELLSALVHVLSSAS
IGNVSSVGVDSTLNVVQDSV
GQYVG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 463 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 749 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MiSp-CT, 1 | 1 |
| 2 | MiSp-CT, 2 | 1 |
Entities:
Entity 1, MiSp-CT, 1 125 residues - 11766.900 Da.
Residues -5 to -1 represent a non-native expression tag
| 1 | GLY | SER | GLY | ASN | SER | VAL | THR | SER | GLY | GLY | ||||
| 2 | TYR | GLY | TYR | GLY | THR | SER | ALA | ALA | ALA | GLY | ||||
| 3 | ALA | GLY | VAL | ALA | ALA | GLY | SER | TYR | ALA | GLY | ||||
| 4 | ALA | VAL | ASN | ARG | LEU | SER | SER | ALA | GLU | ALA | ||||
| 5 | ALA | SER | ARG | VAL | SER | SER | ASN | ILE | ALA | ALA | ||||
| 6 | ILE | ALA | SER | GLY | GLY | ALA | SER | ALA | LEU | PRO | ||||
| 7 | SER | VAL | ILE | SER | ASN | ILE | TYR | SER | GLY | VAL | ||||
| 8 | VAL | ALA | SER | GLY | VAL | SER | SER | ASN | GLU | ALA | ||||
| 9 | LEU | ILE | GLN | ALA | LEU | LEU | GLU | LEU | LEU | SER | ||||
| 10 | ALA | LEU | VAL | HIS | VAL | LEU | SER | SER | ALA | SER | ||||
| 11 | ILE | GLY | ASN | VAL | SER | SER | VAL | GLY | VAL | ASP | ||||
| 12 | SER | THR | LEU | ASN | VAL | VAL | GLN | ASP | SER | VAL | ||||
| 13 | GLY | GLN | TYR | VAL | GLY |
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