BMRB Entry 19591
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19591
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Title: Aliphatic 13C Chemical Shift Assignments For the Intermediate State of the FF Domain L24A Mutant PubMed: 24211467
Deposition date: 2013-10-30 Original release date: 2014-02-11
Authors: Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis
Citation: Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis. "Visualizing side chains of invisible protein conformers by solution NMR." J. Mol. Biol. 426, 763-774 (2014).
Assembly members:
FF_domain_L24A_mutant, polymer, 71 residues, 8194.3 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
FF_domain_L24A_mutant: GSQPAKKTYTWNTKEEAKQA
FKEALKEKRVPSNASWEQAM
KMIINDPRYSALAKLSEKKQ
AFNAYKVQTEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 203 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FF domain L24A mutant, 1 | 1 |
| 2 | FF domain L24A mutant, 2 | 1 |
Entities:
Entity 1, FF domain L24A mutant, 1 71 residues - 8194.3 Da.
| 1 | GLY | SER | GLN | PRO | ALA | LYS | LYS | THR | TYR | THR | ||||
| 2 | TRP | ASN | THR | LYS | GLU | GLU | ALA | LYS | GLN | ALA | ||||
| 3 | PHE | LYS | GLU | ALA | LEU | LYS | GLU | LYS | ARG | VAL | ||||
| 4 | PRO | SER | ASN | ALA | SER | TRP | GLU | GLN | ALA | MET | ||||
| 5 | LYS | MET | ILE | ILE | ASN | ASP | PRO | ARG | TYR | SER | ||||
| 6 | ALA | LEU | ALA | LYS | LEU | SER | GLU | LYS | LYS | GLN | ||||
| 7 | ALA | PHE | ASN | ALA | TYR | LYS | VAL | GLN | THR | GLU | ||||
| 8 | LYS |
Samples:
sample_1: FF domain L24A mutant, [U-100% 13C; U-100% 15N], 2.0 mM; sodium acetate 50 mM; EDTA 0.5 mM; sodium azide 0.02%; sodium chloride 100 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 5.7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D aliphatic 13C CEST | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v7.5 Rev 2012.204.11.07, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
NMR spectrometers:
- Varian INOVA 600 MHz