BMRB Entry 19613
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19613
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title: Structural insights into the DNA recognition and protein interaction domains reveal fundamental homologous DNA pairing properties of HOP2 PubMed: 24711446
Deposition date: 2013-11-13 Original release date: 2014-04-11
Authors: Moktan, Hem; Guiraldelli, Michel; Eyter, Craig; Zhao, Weixing; Camerini-Otero, Rafael; Sung, Patrick; Zhou, Donghua; Pezza, Roberto
Citation: Moktan, Hem; Guiraldelli, Michel; Eyster, Craig; Zhao, Weixing; Lee, Chih-Ying; Mather, Timothy; Camerini-Otero, R. Daniel; Sung, Patrick; Zhou, Donghua; Pezza, Roberto. "Solution Structure and DNA-binding Properties of the Winged Helix Domain of the Meiotic Recombination HOP2 Protein." J. Biol. Chem. ., .-. (2014).
Assembly members:
Hop2, polymer, 84 residues, 7146.221 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
Hop2: MSKSRAEAAAGAPGIILRYL
QEQNRPYSAQDVFGNLQKEH
GLGKAAVVKALDQLAQEGKI
KEKTYGKQKIYFADQNQFDT
VSDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 310 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 485 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HOP2 | 1 |
Entities:
Entity 1, HOP2 84 residues - 7146.221 Da.
| 1 | MET | SER | LYS | SER | ARG | ALA | GLU | ALA | ALA | ALA | ||||
| 2 | GLY | ALA | PRO | GLY | ILE | ILE | LEU | ARG | TYR | LEU | ||||
| 3 | GLN | GLU | GLN | ASN | ARG | PRO | TYR | SER | ALA | GLN | ||||
| 4 | ASP | VAL | PHE | GLY | ASN | LEU | GLN | LYS | GLU | HIS | ||||
| 5 | GLY | LEU | GLY | LYS | ALA | ALA | VAL | VAL | LYS | ALA | ||||
| 6 | LEU | ASP | GLN | LEU | ALA | GLN | GLU | GLY | LYS | ILE | ||||
| 7 | LYS | GLU | LYS | THR | TYR | GLY | LYS | GLN | LYS | ILE | ||||
| 8 | TYR | PHE | ALA | ASP | GLN | ASN | GLN | PHE | ASP | THR | ||||
| 9 | VAL | SER | ASP | ALA |
Samples:
sample_1: Hop2, [U-99% 13C; U-99% 15N], 6 mg/mL; D2O, [U-99% 2H], 5%; imidazole 10 mM; sodium chloride 120 mM; glycerol 5%
sample_conditions_1: ionic strength: 120 mM; pH: 7.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, peak picking
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution
RPF, Huang, Powers, Montelione - refinement
Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - structure validation
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| NCBI | NP_032975.1 |
| PDB | |
| DBJ | BAA23155 BAE21670 |
| GB | AAH30169 ACR24246 EDL03884 |
| REF | NP_032975 XP_005070210 XP_005369097 XP_005369098 XP_012968832 |
| SP | O35047 |
| AlphaFold | O35047 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts