BMRB Entry 19770

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19770

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Title:
Backbone NMR Assignment of Humicola insolens cutinase
Deposition date:
2014-02-06
Original release date:
2021-12-07
Authors:
Wimmer, Reinhard; Kold, David; Svendsen, Allan; Petersen, Evamaria
Citation:

Citation: Kold, David; Dauter, Zbigniew; Laustsen, Anne; Brzozowski, Andrzej; Turkenburg, Johan; Nielsen, Anders; Koldsoe, Heidi; Petersen, Evamaria; Schioett, Birgit; De Maria, Leonardo; Wilson, Keith; Svendsen, Allan; Wimmer, Reinhard. "Thermodynamic and structural investigation of the specific SDS binding of Humicola insolens cutinase"  Protein Sci. 23, 1023-1035 (2014).
PubMed: 24832484

Assembly members:

Assembly members:
cutinase, polymer, 194 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: ascomycetes   Taxonomy ID: 34413   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Humicola insolens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cutinase: XLGAIENGLESGSANACPDA ILIFARGSTEPGNMGITVGP ALANGLESHIRNIWIQGVGG PYDAALATNFLPRGTSQANI DEGKRLFALANQKCPNTPVV AGGYSQGAALIAAAVSELSG AVKEQVKGVALFGYTQNLQN RGGIPNYPRERTKVFCNVGD AVCTGTLIITPAHLSYTIEA RGEAARFLRDRIRA

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts156
1H chemical shifts283

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1cutinase1

Entities:

Entity 1, cutinase 194 residues - Formula weight is not available

Residue GLU 1 was converted into pyroglutamate when expressing the protein in minimal media in E.coli

1   PCALEUGLYALAILEGLUASNGLYLEUGLU
2   SERGLYSERALAASNALACYSPROASPALA
3   ILELEUILEPHEALAARGGLYSERTHRGLU
4   PROGLYASNMETGLYILETHRVALGLYPRO
5   ALALEUALAASNGLYLEUGLUSERHISILE
6   ARGASNILETRPILEGLNGLYVALGLYGLY
7   PROTYRASPALAALALEUALATHRASNPHE
8   LEUPROARGGLYTHRSERGLNALAASNILE
9   ASPGLUGLYLYSARGLEUPHEALALEUALA
10   ASNGLNLYSCYSPROASNTHRPROVALVAL
11   ALAGLYGLYTYRSERGLNGLYALAALALEU
12   ILEALAALAALAVALSERGLULEUSERGLY
13   ALAVALLYSGLUGLNVALLYSGLYVALALA
14   LEUPHEGLYTYRTHRGLNASNLEUGLNASN
15   ARGGLYGLYILEPROASNTYRPROARGGLU
16   ARGTHRLYSVALPHECYSASNVALGLYASP
17   ALAVALCYSTHRGLYTHRLEUILEILETHR
18   PROALAHISLEUSERTYRTHRILEGLUALA
19   ARGGLYGLUALAALAARGPHELEUARGASP
20   ARGILEARGALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks