BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19779

Title: Solution structure of the SGTA N-terminal domain

Deposition date: 2014-02-07 Original release date: 2014-12-01

Authors: Darby, J.; Krysztofinska, E.; Simpson, P.; Isaacson, Rivka

Citation: Darby, J.; Krysztofinska, E.; Simpson, P.; Simon, A.; Sriskandarajah, N.; Bishop, D.; Hale, L.; Alfano, C.; Conte, M.; High, S.; Isaacson, Rivka. "Solution structure of the SGTA dimerisation domain and investigation of its interactions with the ubiquitin-like domains of BAG6 and UBL4A"  PLOS ONE ., .-..

Assembly members:
SMALL_GLUTAMINE-RICH_TETRATRICOPEPTIDE_REPEAT-CON_PROTEIN_ALPHA, polymer, 83 residues, 9228.2247 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46

Entity Sequences (FASTA):
SMALL_GLUTAMINE-RICH_TETRATRICOPEPTIDE_REPEAT-CON_PROTEIN_ALPHA: MAHHHHHHVDDDDKMDNKKR LAYAIIQFLHDQLRHGGLSS DAQESLEVAIQCLETAFGVT VEDSDLALPQTLPEIFEAAA TGK

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts73
1H chemical shifts485

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 11
2SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 21

Entities:

Entity 1, SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 1 83 residues - 9228.2247 Da.

1   METALAHISHISHISHISHISHISVALASP
2   ASPASPASPLYSMETASPASNLYSLYSARG
3   LEUALATYRALAILEILEGLNPHELEUHIS
4   ASPGLNLEUARGHISGLYGLYLEUSERSER
5   ASPALAGLNGLUSERLEUGLUVALALAILE
6   GLNCYSLEUGLUTHRALAPHEGLYVALTHR
7   VALGLUASPSERASPLEUALALEUPROGLN
8   THRLEUPROGLUILEPHEGLUALAALAALA
9   THRGLYLYS

Samples:

sample_1: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.75 mM

sample_2: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.75 mM

sample_3: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.4 mM; SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA 0.4 mM

sample_conditions_1: ionic strength: 250.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1solutionsample_conditions_1
3D HNCACBsample_1solutionsample_conditions_1
3D CBCA(CO)NHsample_1solutionsample_conditions_1
3D HNCOsample_1solutionsample_conditions_1
3D HN(CA)COsample_1solutionsample_conditions_1
3D HBHA(CO)NHsample_1solutionsample_conditions_1
3D H(CCCO)NHsample_1solutionsample_conditions_1
3D (H)CCCONHsample_1solutionsample_conditions_1
3D 1H-15N NOESYsample_1solutionsample_conditions_1
3D HCCH-TOCSYsample_2solutionsample_conditions_1
3D (H)CCH-TOCSYsample_2solutionsample_conditions_1
3D 1H-13C NOESYsample_2solutionsample_conditions_1
3D F1 1H(13Csample_3solutionsample_conditions_1
15N)-reject 13C NOESYsample_3solutionsample_conditions_1

Software:

ARIA vany, Linge, O'Donoghue and Nilges - chemical shift assignment

AutoDep v4.3, pDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMRView vany, Johnson, One Moon Scientific - chemical shift assignment

TALOS vany, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Other Other 950 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

UNP SGTA_HUMAN
PDB
DBJ BAE01321 BAG57017 BAG73729
EMBL CAA11565 CAB39725 CAB43297 CAG38548 CAG47077
GB AAD13117 AAH00390 AAH02989 AAH05165 AAH08885
PIR T08782
REF NP_001181661 NP_003012 XP_001914923 XP_002761612 XP_003277059
SP O43765
AlphaFold Q9BTZ9 O43765

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts