BMRB Entry 19818
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19818
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Title: Murine Neuroglobin, Fe3+ form (metNgB)
Deposition date: 2014-02-26 Original release date: 2015-03-05
Authors: Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian
Citation: Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian. "1H, 15N and 13C Backbone resonance assignments of murine met-Neuroglobin, free and in complex with cyanide." Biomol. NMR Assignments ., .-..
Assembly members:
metNgb, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b-tev
Entity Sequences (FASTA):
metNgb: MERPESELIRQSWRVVSRSP
LEHGTVLFARLFALEPSLLP
LFQYNGRQFSSPEDSLSSPE
FLDHIRKVMLVIDAAVTNVE
DLSSLEEYLTSLGRKHRAVG
VRLSSFSTVGESLLYMLEKS
LGPDFTPATRTAWSRLYGAV
VQAMSRGWDGE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 853 |
| 15N chemical shifts | 280 |
| 1H chemical shifts | 598 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | metNgb | 1 |
Entities:
Entity 1, metNgb 151 residues - Formula weight is not available
| 1 | MET | GLU | ARG | PRO | GLU | SER | GLU | LEU | ILE | ARG | ||||
| 2 | GLN | SER | TRP | ARG | VAL | VAL | SER | ARG | SER | PRO | ||||
| 3 | LEU | GLU | HIS | GLY | THR | VAL | LEU | PHE | ALA | ARG | ||||
| 4 | LEU | PHE | ALA | LEU | GLU | PRO | SER | LEU | LEU | PRO | ||||
| 5 | LEU | PHE | GLN | TYR | ASN | GLY | ARG | GLN | PHE | SER | ||||
| 6 | SER | PRO | GLU | ASP | SER | LEU | SER | SER | PRO | GLU | ||||
| 7 | PHE | LEU | ASP | HIS | ILE | ARG | LYS | VAL | MET | LEU | ||||
| 8 | VAL | ILE | ASP | ALA | ALA | VAL | THR | ASN | VAL | GLU | ||||
| 9 | ASP | LEU | SER | SER | LEU | GLU | GLU | TYR | LEU | THR | ||||
| 10 | SER | LEU | GLY | ARG | LYS | HIS | ARG | ALA | VAL | GLY | ||||
| 11 | VAL | ARG | LEU | SER | SER | PHE | SER | THR | VAL | GLY | ||||
| 12 | GLU | SER | LEU | LEU | TYR | MET | LEU | GLU | LYS | SER | ||||
| 13 | LEU | GLY | PRO | ASP | PHE | THR | PRO | ALA | THR | ARG | ||||
| 14 | THR | ALA | TRP | SER | ARG | LEU | TYR | GLY | ALA | VAL | ||||
| 15 | VAL | GLN | ALA | MET | SER | ARG | GLY | TRP | ASP | GLY | ||||
| 16 | GLU |
Samples:
sample_1: metNgb, [U-100% 13C; U-100% 15N], 0.5 mM; Tris, HCl 50 mM; D2O 10%; H2O 90%; PMSF 0.05 mM; DSS 0.05 mM
sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 700 MHz
- Bruker Avance 700 MHz
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts