BMRB Entry 19821
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19821
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Contursi, Patrizia; Farina, Biancamaria; Pirone, Luciano; Fusco, Salvatore; Russo, Luigi; Bartolucci, Simonetta; Fattorusso, Roberto; Pedone, Emilia. "Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family." Nucleic Acids Res. 42, 5993-6011 (2014).
PubMed: 24682827
Assembly members:
Stf76, polymer, 77 residues, 9355.989 Da.
Natural source: Common Name: not available Taxonomy ID: 45202 Superkingdom: Plasmid Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-30a(+)
Entity Sequences (FASTA):
Stf76: KAKLSLTQLILIRLSNRGCQ
TLEELEEFTQAKREVLLVTL
TRLHQRGVIYRKWRHFSGRK
YREYCLKHRDELADLEH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 77 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 412 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 77 residues - 9355.989 Da.
| 1 | LYS | ALA | LYS | LEU | SER | LEU | THR | GLN | LEU | ILE | ||||
| 2 | LEU | ILE | ARG | LEU | SER | ASN | ARG | GLY | CYS | GLN | ||||
| 3 | THR | LEU | GLU | GLU | LEU | GLU | GLU | PHE | THR | GLN | ||||
| 4 | ALA | LYS | ARG | GLU | VAL | LEU | LEU | VAL | THR | LEU | ||||
| 5 | THR | ARG | LEU | HIS | GLN | ARG | GLY | VAL | ILE | TYR | ||||
| 6 | ARG | LYS | TRP | ARG | HIS | PHE | SER | GLY | ARG | LYS | ||||
| 7 | TYR | ARG | GLU | TYR | CYS | LEU | LYS | HIS | ARG | ASP | ||||
| 8 | GLU | LEU | ALA | ASP | LEU | GLU | HIS |
Samples:
sample_1: Stf76, [U-100% 13C; U-100% 15N], 100-200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0.05 M; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
CARA, Keller and Wuthrich - chemical shift assignment
CS-Rosetta, Shen, Vernon, Baker and Bax - refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks