BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19823

Title: Structure of Lasso Peptide Caulonodin V

Deposition date: 2014-02-28 Original release date: 2015-01-12

Authors: Zimmermann, Marcel; Hegemann, Julian; Xie, Xiulan; Marahiel, Mohamed

Citation: Zimmermann, Marcel; Hegemann, Julian; Xie, Xiulan; Marahiel, Mohamed. "Characterization of caulonodin lasso peptides revealed unprecedented N-terminal residues and a precursor motif essential for peptide maturation"  Chem. Sci. 5, 4032-4043 (2014).

Assembly members:
entity, polymer, 18 residues, 2002.157 Da.

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 366602   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET41A

Entity Sequences (FASTA):
entity: SIGDSGLRESMSSQTYWP

Data sets:
Data typeCount
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lasso Peptide Caulonodin V1

Entities:

Entity 1, Lasso Peptide Caulonodin V 18 residues - 2002.157 Da.

1   SERILEGLYASPSERGLYLEUARGGLUSER
2   METSERSERGLNTHRTYRTRPPRO

Samples:

sample_1: cnd5 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

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