BMRB Entry 19825
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19825
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: HLA-B*35:01 with peptide VPLRAMTY PubMed: 25028510
Deposition date: 2014-03-02 Original release date: 2019-07-12
Authors: Yanaka, Saeko; Sugase, Kenji
Citation: Yanaka, Saeko; Ueno, Takamasa; Yi, Shi; Qi, Jianxun; Gao, George; Tsumoto, Kouhei; Sugase, Kenji. "Peptide-dependent conformational fluctuation determines the stability of the human leukocyte antigen class I complex" J. Biol. Chem. 289, 24680-24690 (2014).
Assembly members:
HLA-B*35:01_with_peptide_VPLRAMTY, polymer, 276 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b
Entity Sequences (FASTA):
HLA-B*35:01_with_peptide_VPLRAMTY: GSHSMRYFYTAMSRPGRGEP
RFIAVGYVDDTQFVRFDSDA
ASPRTEPRPPWIEQEGPEYW
DRNTQIFKTNTQTYRESLRN
LRGYYNQSEAGSHIIQRMYG
CDLGPDGRLLRGHDQSAYDG
KDYIALNEDLSSWTAADTAA
QITQRKWEAARVAEQLRAYL
EGLCVEWLRRYLENGKETLQ
RADPPKTHVTHHPVSDHEAT
LRCWALGFYPAEITLTWQRD
GEDQTQDTELVETRPAGDRT
FQKWAAVVVPSGEEQRYTCH
VQHEGLPKPLTLRWEP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 617 |
| 15N chemical shifts | 213 |
| 1H chemical shifts | 215 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | beta-2-microglobulin | 1 |
Entities:
Entity 1, beta-2-microglobulin 276 residues - Formula weight is not available
| 1 | GLY | SER | HIS | SER | MET | ARG | TYR | PHE | TYR | THR | ||||
| 2 | ALA | MET | SER | ARG | PRO | GLY | ARG | GLY | GLU | PRO | ||||
| 3 | ARG | PHE | ILE | ALA | VAL | GLY | TYR | VAL | ASP | ASP | ||||
| 4 | THR | GLN | PHE | VAL | ARG | PHE | ASP | SER | ASP | ALA | ||||
| 5 | ALA | SER | PRO | ARG | THR | GLU | PRO | ARG | ALA | PRO | ||||
| 6 | TRP | ILE | GLU | GLN | GLU | GLY | PRO | GLU | TYR | TRP | ||||
| 7 | ASP | ARG | ASN | THR | GLN | ILE | PHE | LYS | THR | ASN | ||||
| 8 | THR | GLN | THR | TYR | ARG | GLU | SER | LEU | ARG | ASN | ||||
| 9 | LEU | ARG | GLY | TYR | TYR | ASN | GLN | SER | GLU | ALA | ||||
| 10 | GLY | SER | HIS | ILE | ILE | GLN | ARG | MET | TYR | GLY | ||||
| 11 | CYS | ASP | LEU | GLY | PRO | ASP | GLY | ARG | LEU | LEU | ||||
| 12 | ARG | GLY | HIS | ASP | GLN | SER | ALA | TYR | ASP | GLY | ||||
| 13 | LYS | ASP | TYR | ILE | ALA | LEU | ASN | GLU | ASP | LEU | ||||
| 14 | SER | SER | TRP | THR | ALA | ALA | ASP | THR | ALA | ALA | ||||
| 15 | GLN | ILE | THR | GLN | ARG | LYS | TRP | GLU | ALA | ALA | ||||
| 16 | ARG | VAL | ALA | GLU | GLN | LEU | ARG | ALA | TYR | LEU | ||||
| 17 | GLU | GLY | LEU | CYS | VAL | GLU | TRP | LEU | ARG | ARG | ||||
| 18 | TYR | LEU | GLU | ASN | GLY | LYS | GLU | THR | LEU | GLN | ||||
| 19 | ARG | ALA | ASP | PRO | PRO | LYS | THR | HIS | VAL | THR | ||||
| 20 | HIS | HIS | PRO | VAL | SER | ASP | HIS | GLU | ALA | THR | ||||
| 21 | LEU | ARG | CYS | TRP | ALA | LEU | GLY | PHE | TYR | PRO | ||||
| 22 | ALA | GLU | ILE | THR | LEU | THR | TRP | GLN | ARG | ASP | ||||
| 23 | GLY | GLU | ASP | GLN | THR | GLN | ASP | THR | GLU | LEU | ||||
| 24 | VAL | GLU | THR | ARG | PRO | ALA | GLY | ASP | ARG | THR | ||||
| 25 | PHE | GLN | LYS | TRP | ALA | ALA | VAL | VAL | VAL | PRO | ||||
| 26 | SER | GLY | GLU | GLU | GLN | ARG | TYR | THR | CYS | HIS | ||||
| 27 | VAL | GLN | HIS | GLU | GLY | LEU | PRO | LYS | PRO | LEU | ||||
| 28 | THR | LEU | ARG | TRP | GLU | PRO |
Samples:
sample_1: HLA-B*35:01 with peptide VPLRAMTY, [U-13C; U-15N; U-2H], 800 – 900 uM; potassium phosphate 20 mM; sodium chloride 143 mM; sodium azide 2 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts