BMRB Entry 19845
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19845
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Title: Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins PubMed: 24668816
Deposition date: 2014-03-09 Original release date: 2014-03-31
Authors: Li, Yan; Surya, Wahyu; Claudine, Stephanie; Torres, Jaume
Citation: Li, Yan; Surya, Wahyu; Claudine, Stephanie; Torres, Jaume. "Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins." J. Biol. Chem. 289, 12535-12549 (2014).
Assembly members:
entity, polymer, 58 residues, 6066.473 Da.
Natural source: Common Name: Coronavirus Taxonomy ID: not available Superkingdom: viruses Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTBMalE
Entity Sequences (FASTA):
entity: ETGTLIVNSVLLFLAFVVFL
LVTLAILTALRLAAYAANIV
NVSLVKPTVYVYSRVKNL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 260 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 461 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Coronavirus Envelope Proteins-1 | 1 |
Entities:
Entity 1, Coronavirus Envelope Proteins-1 58 residues - 6066.473 Da.
| 1 | GLU | THR | GLY | THR | LEU | ILE | VAL | ASN | SER | VAL | ||||
| 2 | LEU | LEU | PHE | LEU | ALA | PHE | VAL | VAL | PHE | LEU | ||||
| 3 | LEU | VAL | THR | LEU | ALA | ILE | LEU | THR | ALA | LEU | ||||
| 4 | ARG | LEU | ALA | ALA | TYR | ALA | ALA | ASN | ILE | VAL | ||||
| 5 | ASN | VAL | SER | LEU | VAL | LYS | PRO | THR | VAL | TYR | ||||
| 6 | VAL | TYR | SER | ARG | VAL | LYS | ASN | LEU |
Samples:
SARS_Etr: Coronavirus Envelope Proteins, [U-99% 13C; U-99% 15N], 0.8 mM; DSS 0.1 mM; D2O, [U-99% 2H], 5%; SDS 50 mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | SARS_Etr | isotropic | sample_conditions_1 |
| 3D HNCA | SARS_Etr | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | SARS_Etr | isotropic | sample_conditions_1 |
| 3D HNCO | SARS_Etr | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | SARS_Etr | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | SARS_Etr | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | SARS_Etr | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | SARS_Etr | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - refinement
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB |
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