BMRB Entry 19991

Name: NMR assignement of salt stabilised Yeast Frataxin YFH1.
Published: 2015-10-22

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19991

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignement of salt stabilised Yeast Frataxin YFH1.

Deposition date:

2014-05-28

Original release date:

2015-10-22

Authors:

Vilanova, Bartolome; Sanfelice, Domenico; Martorell, Gabriel; Pastore, Annalisa; Temussi, Piero

Citation:

Citation: Vilanova, Bartolome; Sanfelice, Domenico; Martorell, Gabriel; Temussi, Piero; Pastore, Annalisa. "Trapping a salt-dependent unfolding intermediate of the marginally stable protein Yfh1" Front. Mol. Biosci. ., .-. (2014).
PubMed: 25988154

Assembly members:

Assembly members:
Yeast_Frataxin_Yfh1, polymer, 123 residues, 13783.4 Da.

Natural source:

Natural source: Common Name: E. Coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast_Frataxin_Yfh1: MESSTDGQVVPQEVLNLPLE KYHEEADDYLDHLLDSLEEL SEAHPDCIPDVELSHGVMTL EIPAFGTYVINKQPPNKQIW LASPLSGPNRFDLLNGEWVS LRNGTKLTDILTEEVEKAIS KSQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	486
15N chemical shifts	110
1H chemical shifts	752

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yeast frataxin	1

Entities:

Entity 1, yeast frataxin 123 residues - 13783.4 Da.

1

MET

GLU

SER

THR

ASP

GLY

GLN

VAL

2

PRO

GLN

GLU

VAL

LEU

ASN

LEU

PRO

LEU

GLU

3

LYS

TYR

HIS

GLU

ALA

ASP

TYR

LEU

4

ASP

HIS

LEU

ASP

SER

LEU

GLU

LEU

5

SER

GLU

ALA

HIS

PRO

ASP

CYS

ILE

PRO

ASP

6

VAL

GLU

LEU

SER

HIS

GLY

VAL

MET

THR

LEU

7

GLU

ILE

PRO

ALA

PHE

GLY

THR

TYR

VAL

ILE

8

ASN

LYS

GLN

PRO

ASN

LYS

GLN

ILE

TRP

9

LEU

ALA

SER

PRO

LEU

SER

GLY

PRO

ASN

ARG

10

PHE

ASP

LEU

ASN

GLY

GLU

TRP

VAL

SER

11

LEU

ARG

ASN

GLY

THR

LYS

LEU

THR

ASP

ILE

12

LEU

THR

GLU

VAL

GLU

LYS

ALA

ILE

SER

13

LYS

SER

GLN

Samples:

sample_1: Yeast Frataxin, Yfh1, [U-98% 13C; U-98% 15N], 1 ± 0.1 mM; HEPES 20 mM; NaCl 100 mM; TCEP 2 mM; Yfh1 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

BMRB	17068 17641
PDB	2FQL 2GA5 3OEQ 3OER 4EC2
DBJ	GAA22128
EMBL	CAA98688 CAY78388
GB	AAS56486 AHY74893 AJP37633 AJU57746 AJU58449
REF	NP_010163
SP	Q07540
TPG	DAA11740
AlphaFold	Q07540