BMRB Entry 19999

Name: 1H, 15N, 13C resonance assignment of human osteopontin
Published: 2019-07-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19999

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N, 13C resonance assignment of human osteopontin

Deposition date:

2014-06-02

Original release date:

2019-07-11

Authors:

Platzer, Gerald; Zerko, Szymon; Kozminski, Wiktor; Konrat, Robert

Citation:

Citation: Platzer, Gerald; Zerko, Szymon; Saxena, Saurabh; Kozminski, Wiktor; Konrat, Robert. "1H, 15N, 13C resonance assignment of human osteopontin" Biomol. NMR Assign. 9, 289-292 (2015).
PubMed: 25616494

Assembly members:

Assembly members:
osteopontin, polymer, 302 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-m11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
osteopontin: GAMGIPVKQADSGSSEEKQL YNKYPDAVATWLNPDPSQKQ NLLAPQNAVSSEETNDFKQE TLPSKSNESHDHMDDMDDED DDDHVDSQDSIDSNDSDDVD DTDDSHQSDESHHSDESDEL VTDFPTDLPATEVFTPVVPT VDTYDGRGDSVVYGLRSKSK KFRRPDIQYPDATDEDITSH MESEELNGAYKAIPVAQDLN APSDWDSRGKDSYETSQLDD QSAETHSHKQSRLYKRKAND ESNEHSDVIDSQELSKVSRE FHSHEFHSHEDMLVVDPKSK EEDKHLKFRISHELDSASSE VN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	902
15N chemical shifts	257
1H chemical shifts	1147

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	osteopontin	1

Entities:

Entity 1, osteopontin 302 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ILE

PRO

VAL

LYS

GLN

ALA

2

ASP

SER

GLY

SER

GLU

LYS

GLN

LEU

3

TYR

ASN

LYS

TYR

PRO

ASP

ALA

VAL

ALA

THR

4

TRP

LEU

ASN

PRO

ASP

PRO

SER

GLN

LYS

GLN

5

ASN

LEU

ALA

PRO

GLN

ASN

ALA

VAL

SER

6

SER

GLU

THR

ASN

ASP

PHE

LYS

GLN

GLU

7

THR

LEU

PRO

SER

LYS

SER

ASN

GLU

SER

HIS

8

ASP

HIS

MET

ASP

MET

ASP

GLU

ASP

9

ASP

HIS

VAL

ASP

SER

GLN

ASP

SER

10

ILE

ASP

SER

ASN

ASP

SER

ASP

VAL

ASP

11

ASP

THR

ASP

SER

HIS

GLN

SER

ASP

GLU

12

SER

HIS

SER

ASP

GLU

SER

ASP

GLU

LEU

13

VAL

THR

ASP

PHE

PRO

THR

ASP

LEU

PRO

ALA

14

THR

GLU

VAL

PHE

THR

PRO

VAL

PRO

THR

15

VAL

ASP

THR

TYR

ASP

GLY

ARG

GLY

ASP

SER

16

VAL

TYR

GLY

LEU

ARG

SER

LYS

SER

LYS

17

LYS

PHE

ARG

PRO

ASP

ILE

GLN

TYR

PRO

18

ASP

ALA

THR

ASP

GLU

ASP

ILE

THR

SER

HIS

19

MET

GLU

SER

GLU

LEU

ASN

GLY

ALA

TYR

20

LYS

ALA

ILE

PRO

VAL

ALA

GLN

ASP

LEU

ASN

21

ALA

PRO

SER

ASP

TRP

ASP

SER

ARG

GLY

LYS

22

ASP

SER

TYR

GLU

THR

SER

GLN

LEU

ASP

23

GLN

SER

ALA

GLU

THR

HIS

SER

HIS

LYS

GLN

24

SER

ARG

LEU

TYR

LYS

ARG

LYS

ALA

ASN

ASP

25

GLU

SER

ASN

GLU

HIS

SER

ASP

VAL

ILE

ASP

26

SER

GLN

GLU

LEU

SER

LYS

VAL

SER

ARG

GLU

27

PHE

HIS

SER

HIS

GLU

PHE

HIS

SER

HIS

GLU

28

ASP

MET

LEU

VAL

ASP

PRO

LYS

SER

LYS

29

GLU

ASP

LYS

HIS

LEU

LYS

PHE

ARG

ILE

30

SER

HIS

GLU

LEU

ASP

SER

ALA

SER

GLU

31

VAL

ASN

Samples:

nmr_sample: sodium phosphate 50 mM; sodium chloride 50 mM; osteopontin, [U-95% 13C; U-95% 15N], 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	nmr_sample	isotropic	sample_conditions_1
4D HN(CA)NH	nmr_sample	isotropic	sample_conditions_1
5D HN(CA)CONH	nmr_sample	isotropic	sample_conditions_1
5D (HACA)CON(CA)CONH	nmr_sample	isotropic	sample_conditions_1
5D (H)NCO(NCA)CONH	nmr_sample	isotropic	sample_conditions_1
5D HNCOCACB	nmr_sample	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

Agilent Direct Drive 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks