BMRB Entry 20005
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20005
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Title: NMR Solution Structure of Leucine-Enkephlain in DMPC+CHAPS (1:4) bicelles in 90% H2O/10% D2O of pH 6.6 at 298K PubMed: 18412380
Deposition date: 2007-12-27 Original release date: 2008-05-27
Authors: Mukhopadhyay, Chaitali; Gayen, Anindita
Citation: Gayen, Anindita; Mukhopadhyay, Chaitali. "Evidence for Effect of GM1 on Opioid Peptide Conformation: NMR Study on Leucine Enkephalin in Ganglioside-Containing Isotropic Phospholipid Bicelles" Langmuir 24, 5422-5432 (2008).
Assembly members:
Leu-Enkephalin, polymer, 5 residues, 555.62 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Leu-Enkephalin: YGGFL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 24 |
| 1H chemical shifts | 28 |
| coupling constants | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Leu-enkephalin | 1 |
Entities:
Entity 1, Leu-enkephalin 5 residues - 555.62 Da.
TYROSINE 1 GLYCINE 2 GLYCINE 3 PHENYLALANINE 4 LEUCINE 5
| 1 | TYR | GLY | GLY | PHE | LEU |
Samples:
sample: Leu-Enkephalin 4-5 mM; DMPC+CHAPS+GM1 4 %w/v
bicelles: pH: 6.6 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample | isotropic | bicelles |
| 2D 1H-1H NOESY | sample | isotropic | bicelles |
| 2D DQF-COSY | sample | isotropic | bicelles |
| 2D 1H-13C HSQC | sample | isotropic | bicelles |
Software:
xwinnmr v3.75, Bruker Biospin, Goddard, Schwieters, Kuszewski, Tjandra and Clore - collection, data analysis, refinement
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |