BMRB Entry 20022
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20022
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Title: 1H Chemical Shift Assignments for metastin analog, s5a PubMed: 19275876
Deposition date: 2008-04-17 Original release date: 2011-02-14
Authors: Jae il, Kim
Citation: Lee, Ju Yeon; Moon, Jung Sun; Eu, Young-Jae; Lee, Chul Won; Yang, Sung-Tae; Lee, Seung Kyu; Jung, Hyun Ho; Kim, Ha Hyung; Rhim, Hyewhon; Seong, Jae Young; Kim, Jae Il. "Molecular interaction between kisspeptin decapeptide analogs and a lipid membrane" Arch. Biochem. Biophys. 485, 109-114 (2009).
Assembly members:
GPCR_ligand_metastin_analog, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
GPCR_ligand_metastin_analog: YNWNAFGLRFX
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 61 |
| conformer coordinate sets | 1 |
| coupling constants | 9 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | metastin analog | 1 |
Entities:
Entity 1, metastin analog 11 residues - Formula weight is not available
| 1 | TYR | ASN | TRP | ASN | ALA | PHE | GLY | LEU | ARG | PHE | ||||
| 2 | NH2 |
Samples:
S5A: GPCR_ligand_metastin_analog 1 mM; DPC, [U-100% 2H], 100 mM
sample_conditions_1: pH: 3.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | S5A | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | S5A | isotropic | sample_conditions_1 |
| 2D DQF-COSY | S5A | isotropic | sample_conditions_1 |
Software:
CNS, Brunger - chemical shift calculation
NMR spectrometers:
- Bruker Avance 600 MHz