BMRB Entry 20085

Name: Solution structure of SFT-L1
Published: 2011-03-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20085

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of SFT-L1

Deposition date:

2009-07-21

Original release date:

2011-03-21

Authors:

Mylne, Joshua; Daly, Norelle; Colgrave, Michelle; McCallum, Emily; Jones, Alun; Craik, David

Citation:

Citation: Mylne, Joshua; Colgrave, Michelle; Daly, Norelle; Chanson, Aurelie; Elliott, Alysha; McCallum, Emily; Jones, Alun; Craik, David. "Albumins and their processing machinery are hijacked for cyclic peptides in sunflower" Nat. Chem. Biol. 7, 257-259 (2011).
PubMed: 21423169

Assembly members:

Assembly members:
SFT-L1, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source: Common Name: sunflowers Taxonomy ID: 4232 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Helianthus Helianthus annuus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SFT-L1: GCIEGSPVCFPD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	68

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SFT-L1	1

Entities:

Entity 1, SFT-L1 12 residues - Formula weight is not available

1

GLY

CYS

ILE

GLU

GLY

SER

PRO

VAL

CYS

PHE

2

PRO

ASP

Samples:

sample_1: SFT-L1 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5; temperature: 290 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.1, Bruker Biospin - collection

XEASY, Bartels et al. - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Bruker Avance 600 MHz