BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 2067

Title: Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan

Citation: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan. "Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR"  Biochemistry 30, 11348-11356 (1991).

Assembly members:
barnase, polymer, 110 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:   Production method: not available

Entity Sequences (FASTA):
barnase: AQVINTFDGVADYLQTYHKL PDNYITKSEAQALGWVASKG NLADVAPGKSIGGDIFSNRE GKLPGKSGRTWREADINYTS GFRNSDRILYSSDWLIYKTT DHYQTFTKIR

Data sets:
Data typeCount
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 110 residues - Formula weight is not available

1   ALAGLNVALILEASNTHRPHEASPGLYVAL
2   ALAASPTYRLEUGLNTHRTYRHISLYSLEU
3   PROASPASNTYRILETHRLYSSERGLUALA
4   GLNALALEUGLYTRPVALALASERLYSGLY
5   ASNLEUALAASPVALALAPROGLYLYSSER
6   ILEGLYGLYASPILEPHESERASNARGGLU
7   GLYLYSLEUPROGLYLYSSERGLYARGTHR
8   TRPARGGLUALAASPILEASNTYRTHRSER
9   GLYPHEARGASNSERASPARGILELEUTYR
10   SERSERASPTRPLEUILETYRLYSTHRTHR
11   ASPHISTYRGLNTHRPHETHRLYSILEARG

Samples:

sample_one:

sample_condition_set_one: pH: 4.5 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 16169 16170 16171 16172 2066 2070
PDB
EMBL CAA31365 CBI44445 CCF06756 CCG51396 CDG27389
GB AAA86441 AAB28825 AAB29635 AAC53661 AAP41137
PRF 1204204A 2004243A 721946A
REF WP_003151497 WP_007407419 WP_013353722 WP_015387655 WP_016937826
SP P00648 P35078
AlphaFold P35078 P00648