BMRB Entry 2071
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR2071
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Title: Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan
Citation: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan. "Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR" Biochemistry 30, 11348-11356 (1991).
Assembly members:
barnase, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1390 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus amyloliquefaciens
Experimental source: Production method: not available
Entity Sequences (FASTA):
barnase: XXXXXXFXXVAXXLXXXXXX
XXNXXXKXXXQXXXXXXXXG
XXXXXXXXKXIGGDXFXNXX
XXXXXXXXRTWREADINXXX
GXXNSDRXLXXSXXXIXKTT
XHYQTFXXK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | barnase | 1 |
Entities:
Entity 1, barnase 109 residues - Formula weight is not available
| 1 | X | X | X | X | X | X | PHE | X | X | VAL | ||||
| 2 | ALA | X | X | LEU | X | X | X | X | X | X | ||||
| 3 | X | X | ASN | X | X | X | LYS | X | X | X | ||||
| 4 | GLN | X | X | X | X | X | X | X | X | GLY | ||||
| 5 | X | X | X | X | X | X | X | X | LYS | X | ||||
| 6 | ILE | GLY | GLY | ASP | X | PHE | X | ASN | X | X | ||||
| 7 | X | X | X | X | X | X | X | X | ARG | THR | ||||
| 8 | TRP | ARG | GLU | ALA | ASP | ILE | ASN | X | X | X | ||||
| 9 | GLY | X | X | ASN | SER | ASP | ARG | X | LEU | X | ||||
| 10 | X | SER | X | X | X | ILE | X | LYS | THR | THR | ||||
| 11 | X | HIS | TYR | GLN | THR | PHE | X | X | LYS |
Samples:
sample_one:
sample_condition_set_one: pH: 4.5 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz