BMRB Entry 21060
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21060
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Title: conotoxin Eb1.6 PubMed: 29343689
Deposition date: 2015-12-17 Original release date: 2022-03-01
Authors: Jiang, Ling; Du, Tianpeng
Citation: Liu, Zhuguo; Bartels, Peter; Sadeghi, Mahsa; Du, Tianpeng; Dai, Qing; Zhu, Cui; Yu, Shuo; Wang, Shuo; Dong, Mingxin; Sun, Ting; Guo, Jiabin; Peng, Shuangqing; Jiang, Ling; Adams, David; Dai, Qiuyun. "A novel alpha-conopeptide Eu1.6 inhibits N-type (Ca V 2.2) calcium channels and exhibits potent analgesic activity" Sci. Rep. 8, 1004-1004 (2018).
Assembly members:
entity, polymer, 16 residues, 1669.013 Da.
Natural source: Common Name: Conus eburneus Taxonomy ID: 101300 Superkingdom: Eukaryiota Kingdom: Metazoa Genus/species: Conus eburneus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GCCSNPACMLKNPNLC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Eb1.6 | 1 |
Entities:
Entity 1, Eb1.6 16 residues - 1669.013 Da.
| 1 | GLY | CYS | CYS | SER | ASN | PRO | ALA | CYS | MET | LEU | ||||
| 2 | LYS | ASN | PRO | ASN | LEU | CYS |
Samples:
sample_1: entity 0.5 mM; phosphate buffer 20 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: pH: 5.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 800 MHz