BMRB Entry 21079
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21079
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Title: Influenza Hemagglutinin Peptide
Deposition date: 2017-07-03 Original release date: 2017-11-10
Authors: Caffrey, Michael
Citation: Caffrey, Michael. "Influenza Hemagglutinin Peptide" JBC ., .-..
Assembly members:
entity, polymer, 17 residues, 2020.262 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
entity: WSYNAELLVAMENQHTI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 51 |
| 1H chemical shifts | 102 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Influenza Hemagglutinin Peptide | 1 |
Entities:
Entity 1, Influenza Hemagglutinin Peptide 17 residues - 2020.262 Da.
| 1 | TRP | SER | TYR | ASN | ALA | GLU | LEU | LEU | VAL | ALA | ||||
| 2 | MET | GLU | ASN | GLN | HIS | THR | ILE |
Samples:
sample_1: peptide mM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O10 – 100%; TFE 40%
sample_conditions_1: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Zhengrong and Bax - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMR spectrometers:
- Bruker Avance 900 MHz