BMRB Entry 21099
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21099
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of U3-MYRTX-Tb1a peptide from Ant venom. PubMed: 36410579
Deposition date: 2022-06-17 Original release date: 2022-12-24
Authors: PAQUET, Francoise; TREILHOU, Michel; BARASSE, Valentine
Citation: Barasse, Valentine; Tene, Nathan; Klopp, Christophe; Paquet, Francoise; Tysklind, Niklas; Troispoux, Valerie; Lalague, Hadrien; Orivel, Jerome; Lefranc, Benjamin; Leprince, Jerome; Kenne, Martin; Tindo, Maurice; Treilhou, Michel; Touchard, Axel; Bonnafe, Elsa. "Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins." Insect Biochem. Mol. Biol. 151, 103876-. (2022).
Assembly members:
U3-MYRTX-Tb1a, polymer, 23 residues, Formula weight is not available
Natural source: Common Name: ants Taxonomy ID: 219812 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tetramorium bicarinatum
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
U3-MYRTX-Tb1a: VLPALPLLAGLMSLPFLQHK
LTN
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
13C chemical shifts | 90 |
15N chemical shifts | 21 |
1H chemical shifts | 185 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 23 residues - Formula weight is not available
1 | VAL | LEU | PRO | ALA | LEU | PRO | LEU | LEU | ALA | GLY | ||||
2 | LEU | MET | SER | LEU | PRO | PHE | LEU | GLN | HIS | LYS | ||||
3 | LEU | THR | ASN |
Samples:
sample_1: U3-MYRTX-Tb1a 0.8 ± 0.03 mM; TFE-d2, [U-2H], 100%
sample_conditions_1: pH: 4.6; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.3.1, Linge, O'Donoghue and Nilges - structure solution
CCPNMR v2.1, CCPN - chemical shift assignment, data analysis, peak picking
TOPSPIN v3.6.2, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance III HD 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts