BMRB Entry 25014
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25014
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Title: holo_YqcA_dynamics PubMed: 25093851
Deposition date: 2014-06-12 Original release date: 2014-09-18
Authors: Jin, Changwen; Hu, Yunfei; Ye, Qian
Citation: Ye, Qian; Hu, Yunfei; Jin, Changwen. "Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy." PLoS ONE 9, e103936-e103936 (2014).
Assembly members:
YqcA, polymer, 149 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a(+)
Entity Sequences (FASTA):
YqcA: MAEIGIFVGTMYGNSLLVAE
EAEAILTAQGHKATVFEDPE
LSDWLPYQDKYVLVVTSTTG
QGDLPDSIVPLFQGIKDSLG
FQPNLRYGVIALGDSSYVNF
CNGGKQFDALLQEQSAQRVG
EMLLIDASENPEPETESNPW
VEQWGTLLS
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| heteronuclear NOE values | 135 |
| T1 relaxation values | 136 |
| T2 relaxation values | 136 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YqcA | 1 |
Entities:
Entity 1, YqcA 149 residues - Formula weight is not available
| 1 | MET | ALA | GLU | ILE | GLY | ILE | PHE | VAL | GLY | THR | ||||
| 2 | MET | TYR | GLY | ASN | SER | LEU | LEU | VAL | ALA | GLU | ||||
| 3 | GLU | ALA | GLU | ALA | ILE | LEU | THR | ALA | GLN | GLY | ||||
| 4 | HIS | LYS | ALA | THR | VAL | PHE | GLU | ASP | PRO | GLU | ||||
| 5 | LEU | SER | ASP | TRP | LEU | PRO | TYR | GLN | ASP | LYS | ||||
| 6 | TYR | VAL | LEU | VAL | VAL | THR | SER | THR | THR | GLY | ||||
| 7 | GLN | GLY | ASP | LEU | PRO | ASP | SER | ILE | VAL | PRO | ||||
| 8 | LEU | PHE | GLN | GLY | ILE | LYS | ASP | SER | LEU | GLY | ||||
| 9 | PHE | GLN | PRO | ASN | LEU | ARG | TYR | GLY | VAL | ILE | ||||
| 10 | ALA | LEU | GLY | ASP | SER | SER | TYR | VAL | ASN | PHE | ||||
| 11 | CYS | ASN | GLY | GLY | LYS | GLN | PHE | ASP | ALA | LEU | ||||
| 12 | LEU | GLN | GLU | GLN | SER | ALA | GLN | ARG | VAL | GLY | ||||
| 13 | GLU | MET | LEU | LEU | ILE | ASP | ALA | SER | GLU | ASN | ||||
| 14 | PRO | GLU | PRO | GLU | THR | GLU | SER | ASN | PRO | TRP | ||||
| 15 | VAL | GLU | GLN | TRP | GLY | THR | LEU | LEU | SER |
Samples:
sample_1: YqcA, [U-15N], 1 mM; DTT 20 mM; sodium phosphate 30 mM; sodium chloride 30 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| T1 measurement | sample_1 | isotropic | sample_conditions_1 |
| T2 measurement | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 18154 19151 19152 25013 |
| PDB | |
| DBJ | BAB37073 BAE76862 BAG78574 BAI27051 BAI32080 |
| EMBL | CAP77223 CAQ33114 CAQ99718 CAR04300 CAR09403 |
| GB | AAB40440 AAC75832 AAG57904 AAN44291 AAN81803 |
| REF | NP_311677 NP_417270 NP_708584 WP_000229279 WP_000807726 |
| SP | P65367 P65368 P65369 |
| AlphaFold | P65368 P65367 P65369 |