BMRB Entry 25021
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25021
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Title: Backbone chemical shifts of murine Roquin-1 ROQ domain (147-326), apo form PubMed: 25026077
Deposition date: 2014-06-13 Original release date: 2014-07-01
Authors: Schlundt, Andreas; Sattler, Michael
Citation: Schlundt, Andreas; Heinz, Gitta; Janowski, Robert; Geerlof, Arie; Stehle, Ralf; Heissmeyer, Vigo; Niessing, Dierk; Sattler, Michael. "Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation." Nat. Struct. Mol. Biol. 21, 671-678 (2014).
Assembly members:
ROQ_domain_of_Roquin-1, polymer, 183 residues, 20758.8 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a
Entity Sequences (FASTA):
ROQ_domain_of_Roquin-1: GAMEEEGRIRAMRAARSLGE
RTVTELILQHQNPQQLSSNL
WAAVRARGCQFLGPAMQEEA
LKLVLLALEDGSALSRKVLV
LFVVQRLEPRFPQASKTSIG
HVVQLLYRASCFKVTKRDED
SSLMQLKEEFRTYEALRREH
DSQIVQIAMEAGLRIAPDQW
SSLLYGDQSHKSHMQSIIDK
LQT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 148 |
| 13C chemical shifts | 439 |
| 15N chemical shifts | 148 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ROQ | 1 |
Entities:
Entity 1, ROQ 183 residues - 20758.8 Da.
starts with 147
| 1 | GLY | ALA | MET | GLU | GLU | GLU | GLY | ARG | ILE | ARG | ||||
| 2 | ALA | MET | ARG | ALA | ALA | ARG | SER | LEU | GLY | GLU | ||||
| 3 | ARG | THR | VAL | THR | GLU | LEU | ILE | LEU | GLN | HIS | ||||
| 4 | GLN | ASN | PRO | GLN | GLN | LEU | SER | SER | ASN | LEU | ||||
| 5 | TRP | ALA | ALA | VAL | ARG | ALA | ARG | GLY | CYS | GLN | ||||
| 6 | PHE | LEU | GLY | PRO | ALA | MET | GLN | GLU | GLU | ALA | ||||
| 7 | LEU | LYS | LEU | VAL | LEU | LEU | ALA | LEU | GLU | ASP | ||||
| 8 | GLY | SER | ALA | LEU | SER | ARG | LYS | VAL | LEU | VAL | ||||
| 9 | LEU | PHE | VAL | VAL | GLN | ARG | LEU | GLU | PRO | ARG | ||||
| 10 | PHE | PRO | GLN | ALA | SER | LYS | THR | SER | ILE | GLY | ||||
| 11 | HIS | VAL | VAL | GLN | LEU | LEU | TYR | ARG | ALA | SER | ||||
| 12 | CYS | PHE | LYS | VAL | THR | LYS | ARG | ASP | GLU | ASP | ||||
| 13 | SER | SER | LEU | MET | GLN | LEU | LYS | GLU | GLU | PHE | ||||
| 14 | ARG | THR | TYR | GLU | ALA | LEU | ARG | ARG | GLU | HIS | ||||
| 15 | ASP | SER | GLN | ILE | VAL | GLN | ILE | ALA | MET | GLU | ||||
| 16 | ALA | GLY | LEU | ARG | ILE | ALA | PRO | ASP | GLN | TRP | ||||
| 17 | SER | SER | LEU | LEU | TYR | GLY | ASP | GLN | SER | HIS | ||||
| 18 | LYS | SER | HIS | MET | GLN | SER | ILE | ILE | ASP | LYS | ||||
| 19 | LEU | GLN | THR |
Samples:
sample_1: ROQ domain of Roquin-1, [U-98% 13C; U-98% 15N], 200 – 350 uM; TRIS, pH 7.0 20 mM; TCEP 2 mM; sodium chloride 150 mM
sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 150 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPN_Analysis v2, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| UNP | Q4VGL6 |
| BMRB | 19996 |
| PDB | |
| DBJ | BAC23121 BAD32613 BAE24092 BAG53813 |
| EMBL | CAG07670 CDQ65000 CDQ67780 CDQ69323 |
| GB | AAH68669 AAI36785 AAI38664 AAI44409 AAI52932 |
| REF | NP_001020123 NP_001076328 NP_001084548 NP_001108155 NP_001287779 |
| SP | Q4VGL6 Q5TC82 Q6NUC6 |
| TPG | DAA21174 |
| AlphaFold | Q69Z31 Q5TC82 Q4VGL6 Q6NUC6 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts