BMRB Entry 25044

Name: Hug1 is an Intrinsically Disordered Protein that inhibits RNR activity.
Published: 2019-07-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25044

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Hug1 is an Intrinsically Disordered Protein that inhibits RNR activity. PubMed: 25378334

Deposition date: 2014-06-24 Original release date: 2019-07-11

Authors: Meurisse, Julie; Dagnelie-Bacqin, Agathe; Richet, Nicolas; Charbonnier, Jean-Baptiste; Ochsenbein, Francoise; Peyroche, Anne

Citation: Meurisse, Julie; Bacquin, Agathe; Richet, Nicolas; Charbonnier, Jean-Baptiste; Ochsenbein, Francoise; Peyroche, Anne. "Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit" Nucleic Acids Res. 42, 13174-13185 (2014).

Assembly members:
Hug1, polymer, 73 residues, Formula weight is not available

Natural source: Common Name: bakers yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGST

Entity Sequences (FASTA):
Hug1: AMADPMTMDQGLNPKQFFLD DVVLQDTLCSMSNRVNKSVK TGYLFPKDHVPSANIIAVER RGGLSDIGKNTSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	209
15N chemical shifts	69
1H chemical shifts	314

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hug1	1

Entities:

Entity 1, Hug1 73 residues - Formula weight is not available

1

ALA

MET

ALA

ASP

PRO

MET

THR

MET

ASP

GLN

2

GLY

LEU

ASN

PRO

LYS

GLN

PHE

LEU

ASP

3

ASP

VAL

LEU

GLN

ASP

THR

LEU

CYS

SER

4

MET

SER

ASN

ARG

VAL

ASN

LYS

SER

VAL

LYS

5

THR

GLY

TYR

LEU

PHE

PRO

LYS

ASP

HIS

VAL

6

PRO

SER

ALA

ASN

ILE

ALA

VAL

GLU

ARG

7

ARG

GLY

LEU

SER

ASP

ILE

GLY

LYS

ASN

8

THR

SER

ASN

Samples:

sample_1: Hug1, [U-100% 13C; U-100% 15N], 60 uM; sodium phosphate 20 mM; DSS 0.1 mM; sodium azide 0.1 mM; EDTA 0.1 mM; sodium chloride 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.07 M; pH: 5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts